Extracellular Vesicles Shed by Melanoma Cells Contain a Modified Form of H1.0 Linker Histone and H1.0 mRNA-binding Proteins
| Autor: | Gabriella Schiera, Patrizia Cancemi, A. Fricano, Oriana Colletta, Carlo Maria Di Liegro, Gianluca Di Cara, Veronica Puleo, Italia Di Liegro |
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| Přispěvatelé: | SCHIERA, G, DI LIEGRO, CM, COLLETTA, O, PULEO, V, DI CARA, G, CANCEMI, P, FRICANO, A, DI LIEGRO, I, Schiera, G., DI LIEGRO, C., Puleo, V., Colletta, O., Fricano, A., Cancemi, P., Di Cara, G., DI LIEGRO, I. |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Cancer Research Cellular differentiation Blotting Western Fluorescent Antibody Technique MYEF2 Apoptosis RNA-binding protein exosomes membrane vesicles Real-Time Polymerase Chain Reaction Chromatography Affinity Histones 03 medical and health sciences H1.0 linker histone RNA-binding proteins (RBPs) extracellular vesicles (EVs) membrane vesicles (MVs) Settore BIO/10 - Biochimica Tumor Cells Cultured Humans exosome Secretion RNA Messenger Settore BIO/06 - Anatomia Comparata E Citologia melanoma cell line (A375) myelin expression factor-2 (MYEF2) Melanoma Transcription factor Cell Proliferation biology Reverse Transcriptase Polymerase Chain Reaction EXTRACELLULAR VESICLES RNA-Binding Proteins RNA Cell Differentiation Articles Cell biology Blot Cell Transformation Neoplastic 030104 developmental biology Histone Oncology Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Cancer cell biology.protein |
| Zdroj: | International Journal of Oncology |
| Popis: | Extracellular vesicles (EVs) are shed in the extracellular environment by both prokaryotes and eukaryotes. Although produced from both normal and cancer cells, malignant cells release a much higher amount of EVs, which also contain tumor-specific proteins and RNAs. We previously found that G26/24 oligodendroglioma cells shed EVs that contain the pro-apoptotic factors FasL and TRAIL1-2. Interestingly, G26/24 release, via EVs, extracellular matrix remodelling proteases3, and H1° histone protein4, and mRNA. To shed further light on the role of EVs in discarding proteins and mRNAs otherwise able to counteract proliferative signals, we studied a melanoma cell line (A375). We found that also these cancer cells produce H1° and release it into the extracellular space by EVs. Interestingly, H1° sorted to vesicles has a molecular mass higher than expected, and is probably sumoylated. By T1 RNase-protection assay with the H1° RNA, three main complexes were evidenced in EVs, the most abundant of which has a molecular mass of about 65 kDa. By using a biotinylated H1° RNA to fish interacting factors, we isolated from EVs a few proteins which have been then identified by mass spectrometry: the most abundant is a protein of about 60 kDa: myelin expression factor-2 (MYEF2). Western blot analyses confirmed the presence of MYEF2 in EVs released from A375 melanoma cells. 1. D’Agostino et al. 2006, Int J Oncol 29:1075-85. 2. Lo Cicero A et al. 2011, Int J Oncol 39:1353-57. 3. Lo Cicero A et al. 2012, Matrix Biol 31: 229-33. 4. Schiera G et al. 2013, Int J Oncol 43: 1771-76. |
| Databáze: | OpenAIRE |
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