RNA microarray analysis in prenatal mouse cochlea reveals novel IGF-I target genes: implication of MEF2 and FOXM1 transcription factors

Autor: José G. Pichel, Lourdes Rodriguez-de la Rosa, Hortensia Sánchez-Calderón, Marta Milo, Matthew C. Holley, Isabel Varela-Nieto
Jazyk: angličtina
Rok vydání: 2010
Předmět:
Mef2
lcsh:Medicine
In situ hybridization
Biology
Mice
Gene expression
medicine
Developmental Biology/Developmental Molecular Mechanisms
otorhinolaryngologic diseases
Animals
Inner ear
Insulin-Like Growth Factor I
lcsh:Science
Transcription factor
Cochlea
In Situ Hybridization
DNA Primers
Oligonucleotide Array Sequence Analysis
Mice
Knockout
Developmental Biology/Organogenesis
Multidisciplinary
Base Sequence
Microarray analysis techniques
MEF2 Transcription Factors
Reverse Transcriptase Polymerase Chain Reaction
Gene Expression Profiling
Forkhead Box Protein M1
lcsh:R
Otolaryngology/Ear Pathologies
Forkhead Transcription Factors
Molecular biology
Immunohistochemistry
medicine.anatomical_structure
Myogenic Regulatory Factors
Organ of Corti
Developmental Biology/Cell Differentiation
RNA
lcsh:Q
Signal Transduction
Research Article
Otolaryngology/Audiology
Zdroj: PLoS One
PLoS ONE, Vol 5, Iss 1, p e8699 (2010)
PLoS ONE
Digital.CSIC. Repositorio Institucional del CSIC
instname
ISSN: 1932-6203
Popis: 17 páginas, 8 figuras.-- et al.
[Background]: Insulin-like growth factor-I (IGF-I) provides pivotal cell survival and differentiation signals during inner ear development throughout evolution. Homozygous mutations of human IGF1 cause syndromic sensorineural deafness, decreased intrauterine and postnatal growth rates, and mental retardation. In the mouse, deficits in IGF-I result in profound hearing loss associated with reduced survival, differentiation and maturation of auditory neurons. Nevertheless, little is known about the molecular basis of IGF-I activity in hearing and deafness.
[Methodology/Principal Findings]: A combination of quantitative RT-PCR, subcellular fractionation and Western blotting, along with in situ hybridization studies show IGF-I and its high affinity receptor to be strongly expressed in the embryonic and postnatal mouse cochlea. The expression of both proteins decreases after birth and in the cochlea of E18.5 embryonic Igf1−/− null mice, the balance of the main IGF related signalling pathways is altered, with lower activation of Akt and ERK1/2 and stronger activation of p38 kinase. By comparing the Igf1−/− and Igf1+/+ transcriptomes in E18.5 mouse cochleae using RNA microchips and validating their results, we demonstrate the up-regulation of the FoxM1 transcription factor and the misexpression of the neural progenitor transcription factors Six6 and Mash1 associated with the loss of IGF-I. Parallel, in silico promoter analysis of the genes modulated in conjunction with the loss of IGF-I revealed the possible involvement of MEF2 in cochlear development. E18.5 Igf1+/+ mouse auditory ganglion neurons showed intense MEF2A and MEF2D nuclear staining and MEF2A was also evident in the organ of Corti. At P15, MEF2A and MEF2D expression were shown in neurons and sensory cells. In the absence of IGF-I, nuclear levels of MEF2 were diminished, indicating less transcriptional MEF2 activity. By contrast, there was an increase in the nuclear accumulation of FoxM1 and a corresponding decrease in the nuclear cyclin-dependent kinase inhibitor p27Kip1.
[Conclusions/Significance]: We have defined the spatiotemporal expression of elements involved in IGF signalling during inner ear development and reveal novel regulatory mechanisms that are modulated by IGF-I in promoting sensory cell and neural survival and differentiation. These data will help us to understand the molecular bases of human sensorineural deafness associated to deficits in IGF-I.
This work was partially supported by the Royal Society, DIGNA Biotech, Ministerio de Ciencia e Innovación (BFU2005-00084, SAF2008-00470) and the Fundación Mutua Madrileña. Hortensia Sanchez Calderon and Lourdes Rodriguez-de la Rosa hold contracts from Consejo Superior de Investigaciones Científicas-FSE I3 programme and Centro de Investigación Biomédica en Red de Enfermedades Raras (CIBERER), respectively.
Databáze: OpenAIRE
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