Sample Preprocessing Method for Residual Quinolones in Honey Using Immunoaffinity Resin
| Autor: | Masakazu Horie, Yoshiharu Ihara, Mika Terakawa, Koichi Saito, Tsukasa Kodaira, Hiroyuki Nakazawa, Mihoko Kato, Shinji Itoh |
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| Rok vydání: | 2009 |
| Předmět: |
Chromatography
Elution Chemistry Danofloxacin Phosphate buffered saline Analytic Sample Preparation Methods Honey General Medicine Quinolones Sensitivity and Specificity High-performance liquid chromatography Drug Residues Fluorescence Anti-Bacterial Agents chemistry.chemical_compound Enrofloxacin medicine Agarose Sample preparation Chromatography High Pressure Liquid Food Analysis Norfloxacin medicine.drug |
| Zdroj: | Journal of the Food Hygienic Society of Japan (Shokuhin Eiseigaku Zasshi). 50:135-139 |
| ISSN: | 1882-1006 0015-6426 |
| DOI: | 10.3358/shokueishi.50.135 |
| Popis: | A sample preparation method was developed for determination of quinolones in honey using immunoaffinity resin. For this purpose, an immunoaffinity resin for quinolones was prepared by coupling a quinolone-specific monoclonal antibody to agarose resin. Honey samples diluted with phosphate buffer were reacted with immunoaffinity resin. After the resin was washed, quinolones were eluted with glycine-HCl. Quinolones in the eluate were determined by HPLC with fluorescence detection. No interfering peak was found on the chromatograms of honey samples. The recoveries of quinolones from samples were over 70% at fortification levels of 20 ng/g (for norfloxacin, ciprofloxacin and enrofloxacin) and 10 ng/g (for danofloxacin). The quantification limits of quinolones were 2 ng/g. This sample preprocessing method using immunoaffinity resin was found to be effective and suitable for determining residual quinolones in honey. |
| Databáze: | OpenAIRE |
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