Evidence of the genetic diversity and clonal population structure of Oenococcus oeni strains isolated from different wine-making regions of China
| Autor: | Xiangyuan Wen, Shuwen Liu, Kan Shi, Fangshu Xie, Dongliang Yu, Ling He, Tao Wang |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
China Genotype 030106 microbiology Wine Biology Applied Microbiology and Biotechnology Microbiology Homology (biology) 03 medical and health sciences Intergenic region Typing Amplified Fragment Length Polymorphism Analysis Oenococcus Phylogeny Oenococcus oeni Genetics Genetic diversity Phylogenetic tree Genetic Variation food and beverages General Medicine biology.organism_classification 030104 developmental biology Multilocus sequence typing Amplified fragment length polymorphism Multilocus Sequence Typing |
| Zdroj: | Journal of Microbiology. 56:556-564 |
| ISSN: | 1976-3794 1225-8873 |
| DOI: | 10.1007/s12275-018-7568-7 |
| Popis: | Studies of the genetic diversity and population structure of Oenococcus oeni (O. oeni) strains from China are lacking compared to other countries and regions. In this study, amplified fragment length polymorphism (AFLP) and multilocus sequence typing (MLST) methods were used to investigate the genetic diversity and regional evolutionary patterns of 38 O. oeni strains isolated from different wine-making regions in China. The results indicated that AFLP was markedly more efficient than MLST for typing O. oeni strains. AFLP distinguished 37 DNA patterns compared to 7 sequence types identified using MLST, corresponding to discriminatory indices of 0.999 and 0.602, respectively. The AFLP results revealed a high level of genetic diversity among the O. oeni strains from different regions of China, since two subpopulations and an intraspecific homology higher than 60% were observed. Phylogenetic analysis of the O. oeni strains using the MLST method also identified two major phylogroups, which were differentiated into two distinct clonal complexes by minimum spanning tree analysis. Neither intragenic nor intergenic recombination verified the existence of the clonal population structure of the O. oeni strains. |
| Databáze: | OpenAIRE |
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