Rocket Launcher Mechanism of Collaborative Actin Assembly Defined by Single-Molecule Imaging
| Autor: | Jeffrey P. Bombardier, Richa Jaiswal, Christopher J. Gould, Bruce L. Goode, Jeff Gelles, Dennis Breitsprecher |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Adenomatous Polyposis Coli Protein
Arp2/3 complex macromolecular substances Article Protein filament Profilins Animals Protein Interaction Domains and Motifs Actin Adaptor Proteins Signal Transducing Multidisciplinary biology Actin remodeling Actin cytoskeleton Actins Peptide Fragments Cell biology Actin Cytoskeleton Microscopy Fluorescence Profilin Formins biology.protein Rabbits MDia1 Protein Multimerization Protein Binding |
| Zdroj: | Science. 336:1164-1168 |
| ISSN: | 1095-9203 0036-8075 |
| Popis: | See How They Grow Controlled assembly and disassembly of the actin cytoskeleton is essential for processes such as cell motility, cytokinesis, and tumor metastasis. The formation of new actin filaments appears to involve the protein formin paired with another actin assembly-promoting factor. Breitsprecher et al. (p. 1164 ) used triplecolor single-molecule fluorescence microscopy to visualize actin assembly promoted by the formin, mDia1, and the tumor-suppressor, adenomatous polyposis coli (APC). The two assembly factors interacted directly to initiate filament assembly, after which mDia1 moved with the growing barbed ends while APC remained at the site of nucleation. |
| Databáze: | OpenAIRE |
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