Differential activity of granulocyte-macrophage and macrophage colony stimulating factors on bone resorption in fetal rat long bone organ cultures
| Autor: | Gideon Strassmann, Donald R. Bertolini |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
medicine.medical_specialty
medicine.medical_treatment Immunology Long bone Parathyroid hormone Bone Marrow Cells Granulocyte Biochemistry Bone resorption Bone and Bones Dinoprostone Bone remodeling Organ Culture Techniques Internal medicine medicine Escherichia coli Immunology and Allergy Animals Humans Bone Resorption Molecular Biology Chemistry Macrophage Colony-Stimulating Factor Granulocyte-Macrophage Colony-Stimulating Factor Hematology Colony-stimulating factor Recombinant Proteins Resorption Rats medicine.anatomical_structure Endocrinology Parathyroid Hormone Cell Division Prostaglandin E |
| Zdroj: | Cytokine. 3(5) |
| ISSN: | 1043-4666 |
| Popis: | In this study, the ability of recombinant human macrophage (M) and murine granulocyte-macrophage macrophage (GM) colony stimulating factor (CSF) to affect both basal and stimulated bone resorption in fetal rat long-bone organ cultures was assessed. It was found that M-CSF does not affect basal bone resorption or bone resorption stimulated by parathyroid hormone, recombinant human interleukin 1β, prostaglandin E 2 (PGE 2 ), and 1,25 dihydroxy vitamin D 3 . Specifically, M-CSF at concentrations as high as 30 nM (1 μg/mL) did not modulate 45 Ca release from fetal rat long bones stimulated by these agents. The addition of recombinant murine GM-CSF (at equal molar concentration to M-CSF) also did not affect bone resorption stimulated by parathyroid hormone and interleukin 1β. On the other hand, GM-CSF stimulated basal bone resorption over a 120-h period and augmented the resorption mediated by exogenous PGE 2 over a 48-h incubation. In addition, GM-CSF was shown to stimulate production of endogenous PGE 2 in cultures of bone rudiments. These effects on bone resorption were blocked by the addition of prostaglandin synthesis inhibitors and specific antibodies to murine GM-CSF. These data indicate that M-CSF does not act as a regulator of bone turnover, but GM-CSF may cause bone resorption by stimulating the synthesis of PGE 2 in bone. |
| Databáze: | OpenAIRE |
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