Evolution, structure and membrane association of NDUFAF6, an assembly factor for NADH:ubiquinone oxidoreductase (Complex I)
| Autor: | Bernard D. Lemire |
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| Rok vydání: | 2017 |
| Předmět: |
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Molecular 0301 basic medicine Protein Conformation Cofactor Mitochondrial Proteins 03 medical and health sciences chemistry.chemical_compound Phytoene Oxidoreductase Humans Molecular Biology Gene Phylogeny chemistry.chemical_classification Electron Transport Complex I biology Peripheral membrane protein Cell Biology 030104 developmental biology Mitochondrial respiratory chain chemistry Biochemistry Chaperone (protein) Mitochondrial Membranes biology.protein Molecular Medicine Biogenesis |
| Zdroj: | Mitochondrion. 35:13-22 |
| ISSN: | 1567-7249 |
| DOI: | 10.1016/j.mito.2017.04.005 |
| Popis: | The NADH:ubiquinone oxidoreductase (complex I) is the largest member of the mitochondrial respiratory chain. Its FMN cofactor accepts two electrons from NADH and transfers them to ubiquinone via a chain of iron-sulphur centers. A central core of 14 highly conserved subunits can couple electron transfer to proton translocation. The mammalian enzyme has an additional ~30 accessory subunits. Complex I has important bioenergetic and metabolic functions and is a known source of reactive oxygen species; these functions link it to a number of hereditary and degenerative diseases. For many complex I deficiencies, the primary defect is not in a subunit-encoding gene, but rather in an assembly factor or chaperone that participates in the biogenesis of newly synthesized complex I from individual subunits and cofactors. NDUFAF6 encodes a complex I assembly factor and mutations result in complex I deficiency, Leigh syndrome or Acadian variant Fanconi syndrome. Human NDUFAF6 is a mitochondria-targeted 333-amino acid protein belonging to the family of squalene and phytoene synthases. Sequence and structural information suggests that NDUFAF6 likely has enzymatic activity, but one that has evolved considerable differences from canonical squalene and phytoene synthases. Most but not all metazoans have an NDUFAF6 ortholog, indicating that in some organisms, complex I biogenesis does not require this protein. NDUFAF6 is a peripheral membrane protein and predictions identify a conserved C-terminal attachment site that have implications for substrate access. |
| Databáze: | OpenAIRE |
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