Enhancement of immune response towards non-lipidized Borrelia burgdorferi recombinant OspC antigen by binding onto the surface of metallochelating nanoliposomes with entrapped lipophilic derivatives of norAbuMDP
| Autor: | Eliška Bartheldyová, Milan Raska, Jana Plocková, Pavlína Turánek Knotigová, Marek Šebela, Josef Mašek, Miroslav Ledvina, Pavel Kulich, Ondřej Strouhal, Michal Křupka, Andrew D. Miller, Štěpán Koudelka, Jaroslav Turánek, Lýdie Czerneková, Kateřina Zachová, Milada Horynová, Zina Korvasová, Michaela Škrabalová |
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| Rok vydání: | 2011 |
| Předmět: |
Light
medicine.medical_treatment Pharmaceutical Science chemical and pharmacologic phenomena Enzyme-Linked Immunosorbent Assay Microbiology Mice Immune system Antigen Microscopy Electron Transmission Borrelia parasitic diseases medicine Animals Scattering Radiation Borrelia burgdorferi Chelating Agents Antigens Bacterial Drug Carriers Mice Inbred BALB C biology Calorimetry Differential Scanning Lyme Disease Vaccines bacterial infections and mycoses biology.organism_classification Virology Antibodies Bacterial Immunization Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Liposomes biology.protein Nanoparticles Electrophoresis Polyacrylamide Gel Female Antibody Cell activation Adjuvant Acetylmuramyl-Alanyl-Isoglutamine Bacterial Outer Membrane Proteins |
| Zdroj: | Journal of controlled release : official journal of the Controlled Release Society. 160(2) |
| ISSN: | 1873-4995 |
| Popis: | Lyme disease caused by spirochete Borrelia burgdorferi sensu lato, is a tick-born illness. If the infection is not eliminated by the host immune system and/or antibiotics, it may further disseminate and cause severe chronic complications. The immune response to Borrelia is mediated by phagocytic cells and by Borrelia-specific complement-activating antibodies associated with Th1 cell activation. A new experimental vaccine was constructed using non-lipidized form of recombinant B. burgdorferi s.s. OspC protein was anchored by metallochelating bond onto the surface of nanoliposomes containing novel nonpyrogenic lipophilized norAbuMDP analogues denoted MT05 and MT06. After i.d. immunization, the experimental vaccines surpassed Alum with respect to OspC-specific titers of IgG2a, IgG2b isotypes when MT06 was used and IgG3, IgM isotypes when MT05 was used. Both adjuvants exerted a high adjuvant effect comparable or better than MDP and proved themselves as nonpyrogenic. |
| Databáze: | OpenAIRE |
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