Prevalence of heterotypic tumor/immune cell-in-cell structure in vitro and in vivo leading to formation of aneuploidy
| Autor: | Xiao-juan Che, Ying Wang, Shan Wang, Meifang He, Yanyi Wang, Xiao-min Yu, Jian Ma, Ju-fang Wang, Hua Zhao, Han-yu Zhu, Yuhui Chen, Xiaoning Wang |
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| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Cell Physiology
Anatomy and Physiology T-Lymphocytes Antigen presentation Mitosis lcsh:Medicine Cell Communication Biology Giant Cells Mice Immune system Antigen Neoplasms Molecular Cell Biology Basic Cancer Research Tumor Cells Cultured Animals Humans Killer Cells Lymphokine-Activated lcsh:Science B-Lymphocytes Multidisciplinary Lymphokine-activated killer cell Innate immune system Stem Cells Carcinoma lcsh:R Lymphokine Cancers and Neoplasms Cell Differentiation Acquired immune system Aneuploidy Cellular Structures Cell biology B-1 cell Killer Cells Natural Cell Transformation Neoplastic Oncology Hepatocytes Medicine lcsh:Q Research Article |
| Zdroj: | PLoS ONE, Vol 8, Iss 3, p e59418 (2013) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | Cell-in-cell structures refer to a unique phenomenon that one living cell enters into another living cell intactly, occurring between homotypic tumor cells or tumor (or other tissue cells) and immune cells (named as heterotypic cell-in-cell structure). In the present study, through a large scale of survey we observed that heterotypic cell-in-cell structure formation occurred commonly in vitro with host cells derived from different human carcinomas as well as xenotypic mouse tumor cell lines. Most of the lineages of human immune cells, including T, B, NK cells, monocytes as well as in vitro activated LAK cells, were able to invade tumor cell lines. Poorly differentiated stem cells were capable of internalizing immune cells as well. More significantly, heterotypic tumor/immune cell-in-cell structures were observed in a higher frequency in tumor-derived tissues than those in adjacent tissues. In mouse hepatitis models, heterotypic immune cell/hepatocyte cell-in-cell structures were also formed in a higher frequency than in normal controls. After in vitro culture, different forms of internalized immune cells in heterotypic cell-in-cell structures were observed, with one or multiple immune cells inside host cells undergoing resting, degradation or mitosis. More strikingly, some internalized immune cells penetrated directly into the nucleus of target cells. Multinuclear cells with aneuploid nucleus were formed in target tumor cells after internalizing immune cells as well as in situ tumor regions. Therefore, with the prevalence of heterotypic cell-in-cell structures observed, we suggest that shielding of immune cells inside tumor or inflammatory tissue cells implies the formation of aneuploidy with the increased multinucleation as well as fine-tuning of microenvironment under pathological status, which may define distinct mechanisms to influence the etiology and progress of tumors. |
| Databáze: | OpenAIRE |
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