Effects of Weight Loss and Moderate-Protein, High-Fiber Diet Consumption on the Fasted Serum Metabolome of Cats
| Autor: | Kirk L. Pappan, Patrícia Massae Oba, Maria R C de Godoy, Kelly S. Swanson, Marissa R. Pallotto, Preston R. Buff |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty feline obesity Endocrinology Diabetes and Metabolism Metabolite 030209 endocrinology & metabolism Biochemistry Microbiology Article 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Insulin resistance Blood serum Metabolomics Weight loss Internal medicine medicine Metabolome Molecular Biology CATS business.industry medicine.disease metabolomics QR1-502 030104 developmental biology Endocrinology feline metabolism chemistry Ketone bodies medicine.symptom business |
| Zdroj: | Metabolites Volume 11 Issue 5 Metabolites, Vol 11, Iss 324, p 324 (2021) |
| ISSN: | 2218-1989 |
| DOI: | 10.3390/metabo11050324 |
| Popis: | Feline obesity elicits a plethora of metabolic responses leading to comorbidities, with potential reversal during weight loss. The specific metabolic alterations and biomarkers of organ dysfunction are not entirely understood. Untargeted, high-throughput metabolomic technologies may allow the identification of biological components that change with weight status in cats, increasing our understanding of feline metabolism. The objective of this study was to utilize untargeted metabolomic techniques to identify biomarkers and gain mechanistic insight into the serum metabolite changes associated with reduced food intake and weight loss in overweight cats. During a four-wk baseline period, cats were fed to maintain body weight. For 18 wk following baseline, cats were fed to lose weight at a rate of ~1.5% body weight/wk. Blood serum metabolites were measured at wk 0, 1, 2, 4, 8, 12, and 16. A total of 535 named metabolites were identified, with up to 269 of them being altered (p- and q- values < 0.05) at any time point. A principal component analysis showed a continual shift in metabolite profile as weight loss progressed, with early changes being distinct from those over the long term. The majority of lipid metabolites decreased with weight loss however, ketone bodies and small lipid particles increased with weight loss. The majority of carbohydrate metabolites decreased with weight loss. Protein metabolites had a variable result, with some increasing, but others decreasing with weight loss. Metabolic mediators of inflammation, oxidative stress, xenobiotics, and insulin resistance decreased with weight loss. In conclusion, global metabolomics identified biomarkers of reduced food intake and weight loss in cats, including decreased markers of inflammation and/or altered macronutrient metabolism. |
| Databáze: | OpenAIRE |
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