Antibiotic resistance genes in swine manure slurry as affected by pit additives and facility disinfectants
| Autor: | Shannon L. Bartelt-Hunt, John E. Gilley, Daniel D. Snow, Maria C. Hall, Amy M. Schmidt, Xu Li, Jon Duerschner, Kent M. Eskridge |
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| Rok vydání: | 2020 |
| Předmět: |
Environmental Engineering
010504 meteorology & atmospheric sciences Swine Disinfectant Manure storage 010501 environmental sciences Positive correlation 01 natural sciences stomatognathic system RNA Ribosomal 16S Environmental Chemistry Animals Food science Waste Management and Disposal 0105 earth and related environmental sciences Chemistry Drug Resistance Microbial biochemical phenomena metabolism and nutrition 16S ribosomal RNA Pollution Manure Anti-Bacterial Agents Genes Bacterial Bench scale Slurry Antibiotic resistance genes Disinfectants |
| Zdroj: | The Science of the total environment. 761 |
| ISSN: | 1879-1026 |
| Popis: | Manure storage facilities are critical control points to reduce antibiotic resistance genes (ARGs) in swine manure slurry before the slurry is land applied. However, little is known about how exogenous chemicals entering the manure storage facilities may affect the fate of ARGs. The objective of this study was to analyze the impact of six commonly used pit additives and four facility disinfectants on the concentration of ARGs in swine manure slurry. Bench scale reactors, each containing approximately 50 L of liquid swine manure, were dosed with additives or disinfectants and were sampled for 40 days. Seven antibiotic resistance genes along with the intI1 gene and the 16S rRNA gene were monitored. Out of the six additives tested, Sludge Away significantly reduced the time-averaged absolute abundance of erm(C), erm(F), tet(Q), and the 16S rRNA gene as compared to the no additive control. Out of the four disinfectants tested, Tek-Trol significantly reduced the time-averaged absolute abundance of erm(B), erm(C), erm(F), intI1, tet(Q), and tet(X) than did the no-disinfectant control. According to Spearman's rank correlation, three genes erm(F), tet(Q), and tet(X) showed a strong to perfectly positive correlation and the two genes erm(B) and tet(O) showed a moderate to strong correlation in both the additive and disinfectant tests. Overall, the disinfectants were more effective in controlling the absolute abundance of ARGs than were the pit additives. |
| Databáze: | OpenAIRE |
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