FIV infection of macrophages: in vitro and in vivo inhibition by dideoxycytidine 5′-triphosphate
| Autor: | Fulvia Baldinotti, Lucia Silvotti, Alessandra Fraternale, Mauro Bendinelli, Mauro Magnani, Giuseppe Piedimonte, Donatella Matteucci, L. Rossi, F. Quintavalla |
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| Rok vydání: | 1995 |
| Předmět: |
Male
Feline immunodeficiency virus Erythrocytes viruses Immunology Cell Immunodeficiency Virus Feline Virus Replication Antiviral Agents In vivo Feline Acquired Immunodeficiency Syndrome medicine Animals Lymphocytes Drug Carriers CATS General Veterinary biology Macrophages biology.organism_classification Virology In vitro Specific Pathogen-Free Organisms medicine.anatomical_structure Deoxycytosine Nucleotides Cats Macrophages Peritoneal biology.protein Phosphorylation Female Antibody Nucleoside Dideoxynucleotides |
| Zdroj: | Veterinary Immunology and Immunopathology. 46:151-158 |
| ISSN: | 0165-2427 |
| DOI: | 10.1016/0165-2427(94)07014-x |
| Popis: | We have evaluated in vitro and in vivo whether it is possible to protect cat macrophages from feline immunodeficiency virus (FIV) infection by the administration of dideoxycytidine 5'-triphosphate (DDCTP). Since cell membranes are impermeable to phosphorylated drugs we have encapsulated DDCTP into autologous erythrocytes and modified erythrocyte membranes to target these drug-loaded cells to macrophages. DDCTP-loaded erythrocytes reduced FIV production by macrophages infected in vitro or obtained from naturally or experimentally infected cats. The same treatment protected the majority of peritoneal macrophages during a 7 month experimental FIV infection and reduced the percentage of circulating lymphocytes stained with an anti-p24 antibody. These results suggest that the administration of nucleoside analogues in phosphorylated form is feasible and their targeting to macrophages reduces FIV infection in vitro and in vivo. |
| Databáze: | OpenAIRE |
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