Primary Human mDC1, mDC2, and pDC Dendritic Cells Are Differentially Infected and Activated by Respiratory Syncytial Virus
| Autor: | Kizzmekia S. Corbett, Teresa R. Johnson, Barney S. Graham, Gretchen C. Edwards, Christina N. Johnson |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Chemokine
Viral Diseases medicine.medical_treatment Immune Cells Immunology lcsh:Medicine Antigen-Presenting Cells Respiratory Syncytial Virus Infections Biology Microbiology Virus Green fluorescent protein Flow cytometry Immunophenotyping Immune Activation Immune System Phenomena Multiplicity of infection Immunity Virology medicine Humans lcsh:Science Immunity to Infections Immune Response Cells Cultured Respiratory Syncytial Virus Infection Tissue Inhibitor of Metalloproteinase-2 Multidisciplinary medicine.diagnostic_test Viral Immune Evasion lcsh:R Immune Defense Cell Differentiation Dendritic Cells Innate Immunity Respiratory Syncytial Viruses Host-Pathogen Interaction Cytokine Infectious Diseases Viral replication biology.protein Medicine Cytokines lcsh:Q Research Article |
| Zdroj: | PLoS ONE PLoS ONE, Vol 6, Iss 1, p e16458 (2011) |
| ISSN: | 1932-6203 |
| Popis: | Respiratory syncytial virus (RSV) causes recurrent infections throughout life. Vaccine development may depend upon understanding the molecular basis for induction of ineffective immunity. Because dendritic cells (DCs) are critically involved in early responses to infection, their interaction with RSV may determine the immunological outcome of RSV infection. Therefore, we investigated the ability of RSV to infect and activate primary mDCs and pDCs using recombinant RSV expressing green fluorescent protein (GFP). At a multiplicity of infection of 5, initial studies demonstrated ∼6.8% of mDC1 and ∼0.9% pDCs were infected. We extended these studies to include CD1c(-)CD141(+) mDC2, finding mDC2 infected at similar frequencies as mDC1. Both infected and uninfected cells upregulated phenotypic markers of maturation. Divalent cations were required for infection and maturation, but maturation did not require viral replication. There is evidence that attachment and entry/replication processes exert distinct effects on DC activation. Cell-specific patterns of RSV-induced maturation and cytokine production were detected in mDC1, mDC2, and pDC. We also demonstrate for the first time that RSV induces significant TIMP-2 production in all DC subsets. Defining the influence of RSV on the function of selected DC subsets may improve the likelihood of achieving protective vaccine-induced immunity. |
| Databáze: | OpenAIRE |
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