Trade-Off Relation between Fungicide Sensitivity and Melanin Biosynthesis in Plant Pathogenic Fungi
| Autor: | Hiroyuki Daimon, Ken Harata, Tetsuro Okuno |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Appressorium Multidisciplinary biology Mutant Colletotrichum orbiculare food and beverages 02 engineering and technology Fungus Mycology 021001 nanoscience & nanotechnology biology.organism_classification Article Microbiology Fungicide 03 medical and health sciences 030104 developmental biology Disease management (agriculture) Genetics lcsh:Q 0210 nano-technology Agricultural Science lcsh:Science Gene |
| Zdroj: | iScience, Vol 23, Iss 11, Pp 101660-(2020) iScience |
| ISSN: | 2589-0042 |
| Popis: | Summary Circumventing the emergence of fungicide-resistant strains is a crucial issue for robust disease management in agriculture. The agricultural fungicide ferimzone has been used for the control of rice diseases including rice blast. The emergence of ferimzone-resistant strains in rice fields has not been reported. Here, we identified the copper transport CoICT1 gene as the ferimzone sensitivity gene in Colletotrichum orbiculare and the rice blast fungus Magnaporthe oryzae. Genetic and cytological analyses showed that functional defects in the copper transport pathways, consisting of CoIct1 and P-type ATPase CoCcc2, led to the low sensitivity to ferimzone and the pathogenicity defect due to attenuated melanization in the appressorium. Importantly, the presence of CuSO4 induced high sensitivity to ferimzone even in the coict1 mutant. Our study shows that there is a trade-off relation between the sensitivity to ferimzone and fungal pathogenicity. Graphical Abstract Highlights • Fungal copper transporters, Ict1 and Ccc2, are involved in ferimzone sensitivity • Melanin biosynthesis requires a laccase activity instigated by Ict1-mediated copper • A metal-binding site in Ict1 is crucial for ferimzone sensitivity and pathogenicity • CuSO4 has an enhancing effect on ferimzone sensitivity Genetics; Mycology; Agricultural Science |
| Databáze: | OpenAIRE |
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