Relationship between energy balance and metabolic profiles in plasma and milk of dairy cows in early lactation
| Autor: | Edoardo Saccenti, Wei Xu, Ariette T.M. van Knegsel, Bas Kemp, Renny J. van Hoeij, Jacques Vervoort |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
mammary gland
Mammary gland Energy balance Biochemie Lactose Biochemistry liquid chromatography–mass spectrometry 03 medical and health sciences chemistry.chemical_compound Animal science Metabolomics Pregnancy Lactation Genetics medicine Animals Systems and Synthetic Biology Adaptatiefysiologie 030304 developmental biology VLAG 0303 health sciences Systeem en Synthetische Biologie 3-Hydroxybutyric Acid Body Weight 0402 animal and dairy science food and beverages 04 agricultural and veterinary sciences Metabolism 040201 dairy & animal science metabolomics Diet nuclear magnetic resonance medicine.anatomical_structure Glucose Milk chemistry Gluconeogenesis Adipose Tissue Metabolome WIAS Adaptation Physiology Animal Science and Zoology Composition (visual arts) Cattle Female Energy Metabolism Food Science |
| Zdroj: | Journal of Dairy Science 103 (2020) Journal of Dairy Science, 103, 4795-4805 |
| ISSN: | 0022-0302 |
| Popis: | Negative energy balance in dairy cows in early lactation is related to alteration of metabolic status. However, the relationships among energy balance, metabolic profile in plasma, and metabolic profile in milk have not been reported. In this study our aims were: (1) to reveal the metabolic profiles of plasma and milk by integrating results from nuclear magnetic resonance (NMR) with data from liquid chromatography triple quadrupole mass spectrometry (LC-MS); and (2) to investigate the relationship between energy balance and the metabolic profiles of plasma and milk. For this study 24 individual dairy cows (parity 2.5 ± 0.5; mean ± standard deviation) were studied in lactation wk 2. Body weight (mean ± standard deviation; 627.4 ± 56.4 kg) and milk yield (28.1 ± 6.7 kg/d; mean ± standard deviation) were monitored daily. Milk composition (fat, protein, and lactose) and net energy balance were calculated. Plasma and milk samples were collected and analyzed using LC-MS and NMR. From all plasma metabolites measured, 27 were correlated with energy balance. These plasma metabolites were related to body reserve mobilization from body fat, muscle, and bone; increased blood flow; and gluconeogenesis. From all milk metabolites measured, 30 were correlated with energy balance. These milk metabolites were related to cell apoptosis and cell proliferation. Nine metabolites detected in both plasma and milk were correlated with each other and with energy balance. These metabolites were mainly related to hyperketonemia; β-oxidation of fatty acids; and one-carbon metabolism. The metabolic profiles of plasma and milk provide an in-depth insight into the physiological pathways of dairy cows in negative energy balance in early lactation. In addition to the classical indicators for energy balance (e.g., β-hydroxybutyrate, acetone, and glucose), the current study presents some new metabolites (e.g., glycine in plasma and milk; kynurenine, panthothenate, or arginine in plasma) in lactating dairy cows that are related to energy balance and may be of interest as new indicators for energy balance. |
| Databáze: | OpenAIRE |
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