Clinically relevant behavioral endpoints in a recurrent nitroglycerin migraine model in rats
| Autor: | Todd A. Smitherman, Ainslee P. Johnson, Rachel E. Davis, Morgan E. Davis, Kenneth J. Sufka, Stephanie M. Staszko |
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| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty Neurology genetic structures Photophobia Migraine Disorders Vasodilator Agents medicine.medical_treatment Clinical Neurology Motor Activity Nitroglycerin 03 medical and health sciences 0302 clinical medicine medicine Animals Saline Migraine Behavior Animal Sumatriptan business.industry Headache General Medicine Single injection Serotonin 5-HT1 Receptor Agonists Translational research medicine.disease eye diseases Rats Animal models Disease Models Animal Treatment Outcome 030104 developmental biology Anesthesiology and Pain Medicine Anesthesia Neurology (clinical) medicine.symptom business Hypoactivity Weight gain 030217 neurology & neurosurgery Research Article medicine.drug |
| Zdroj: | The Journal of Headache and Pain |
| ISSN: | 1129-2377 1129-2369 |
| DOI: | 10.1186/s10194-016-0624-y |
| Popis: | Background This research sought to further validate the rat nitroglycerin (NTG) migraine model by comparing the effects of single versus recurrent NTG episodes on behavioral endpoints that mirror ICHD-3 diagnostic criteria for migraine, and to determine if the altered behavioral endpoints are reduced after administration of sumatriptan. Methods Separate cohorts of rats were administered NTG (10 mg/kg/2 ml) or saline (Experiment 1: single injection; Experiment 2: repeated injections; Experiment 3: repeated injections with sumatriptan [0.0, 0.3 and 1.0 mg/kg/ml] rescue. Behavioral endpoints were assessed 2 h after final NTG administration and included time in light/dark chambers for photophobia and activity, pain facial ratings, and cool (5 °C) and warm (46 °C) tail dip. Results The first two experiments demonstrated that repeated (n = 5) but not single NTG injections produced photophobia, decreased activity, and yielded less weight gain than saline injections. Experiment 3 showed that sumatriptan attenuated hypoactivity, reduced facial expressions of pain, and reversed weight alterations in a dose-dependent manner. Conclusions These findings identify numerous clinical homologies of a recurrent NTG rat migraine model that may be useful for screening novel pharmacotherapies. |
| Databáze: | OpenAIRE |
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