Pan-genomic analysis of Corynebacterium amycolatum gives insights into molecular mechanisms underpinning the transition to a pathogenic phenotype

Autor: Jesus, Hendor N. R., Rocha, Danilo J. P. G., Ramos, Rommel T. J., Silva, Artur, Brenig, Bertram, Goes-Neto, Aristoteles, Costa, Mateus M., Soares, Siomar C., Azevedo, Vasco, Aguiar, Eric R. G. R., Martinez-Martinez, Luiz, Ocampo, Alain, Alibi, Sana, Dorta, Alexis, Pacheco, Luis G. C., Navas, Jesus
Přispěvatelé: [Jesus, Hendor N. R.] Univ Fed Bahia, Inst Hlth Sci, Multictr Postgrad Program Biochem & Mol Biol PMBq, Salvador, BA, Brazil, [Pacheco, Luis G. C.] Univ Fed Bahia, Inst Hlth Sci, Multictr Postgrad Program Biochem & Mol Biol PMBq, Salvador, BA, Brazil, [Rocha, Danilo J. P. G.] Univ Fed Bahia, Inst Hlth Sci, Postgrad Program Biotechnol, Salvador, BA, Brazil, [Pacheco, Luis G. C.] Univ Fed Bahia, Inst Hlth Sci, Postgrad Program Biotechnol, Salvador, BA, Brazil, [Ramos, Rommel T. J.] Fed Univ Para, Inst Biol Sci, Belem, Para, Brazil, [Silva, Artur] Fed Univ Para, Inst Biol Sci, Belem, Para, Brazil, [Brenig, Bertram] Univ Gottingen, Inst Vet Med, Gottingen, Germany, [Goes-Neto, Aristoteles] Univ Fed Minas Gerais, Inst Biol Sci, Belo Horizonte, MG, Brazil, [Azevedo, Vasco] Univ Fed Minas Gerais, Inst Biol Sci, Belo Horizonte, MG, Brazil, [Costa, Mateus M.] Univ Fed Vale Do Sao Francisco, Lab Microbiol & Imunol Anim LAMIA, Petrolina, PE, Brazil, [Soares, Siomar C.] Fed Univ Triangulo Mineiro UFTM, Inst Biol & Nat Sci, Dept Immunol Microbiol & Parasitol, Uberaba, MG, Brazil, [Aguiar, Eric R. G. R.] State Univ St Cruz, Dept Biol Sci, Ilheus, BA, Brazil, [Martinez-Martinez, Luiz] Hosp Univ Reina Sofia, Unidad Gest Clin, Cordoba, Spain, [Martinez-Martinez, Luiz] Univ Cordoba, Dept Microbiol, Cordoba, Spain, [Martinez-Martinez, Luiz] Inst Maimonides Invest Biomed Cordoba IMIBIC, Cordoba, Spain, [Ocampo, Alain] Univ Hosp Marques de Valdecilla, Microbiol Serv, Santander, Spain, [Ocampo, Alain] Inst Invest Valdecilla IDIVAL, Santander, Spain, [Dorta, Alexis] Inst Invest Valdecilla IDIVAL, Santander, Spain, [Navas, Jesus] Inst Invest Valdecilla IDIVAL, Santander, Spain, [Alibi, Sana] Res Unit Anal & Proc Appl Environm, Rejiche, Tunisia, [Dorta, Alexis] Cantabria Univ, Fac Med, BIOMEDAGE Grp, Santander, Spain, [Navas, Jesus] Cantabria Univ, Fac Med, BIOMEDAGE Grp, Santander, Spain, FAPESB, CNPq, CAPES, RECOM Network, Brazil
Rok vydání: 2022
Předmět:
Zdroj: Frontiers in Microbiology. 13
ISSN: 1664-302X
Popis: Corynebacterium amycolatum is a nonlipophilic coryneform which is increasingly being recognized as a relevant human and animal pathogen showing multidrug resistance to commonly used antibiotics. However, little is known about the molecular mechanisms involved in transition from colonization to the MDR invasive phenotype in clinical isolates. In this study, we performed a comprehensive pan-genomic analysis of C. amycolatum, including 26 isolates from different countries. We obtained the novel genome sequences of 8 of them, which are multidrug resistant clinical isolates from Spain and Tunisia. They were analyzed together with other 18 complete or draft C. amycolatum genomes retrieved from GenBank. The species C. amycolatum presented an open pan-genome (α = 0.854905), with 3,280 gene families, being 1,690 (51.52%) in the core genome, 1,121 related to accessory genes (34.17%), and 469 related to unique genes (14.29%). Although some classic corynebacterial virulence factors are absent in the species C. amycolatum, we did identify genes associated with immune evasion, toxin, and antiphagocytosis among the predicted putative virulence factors. Additionally, we found genomic evidence for extensive acquisition of antimicrobial resistance genes through genomic islands.
Databáze: OpenAIRE
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