Role of epstein-barr virus in the severity of recurrent respiratory papillomatosis
| Autor: | Estela Kaminagakura, Vivian Narana Ribeiro El-Achkar, Jorge Esquiche León, Barbara Pereira Mello, Shirley Shizue Nagata Pignatari, Alfredo Ribeiro-Silva, Patrícia Pimentel de Barros, Laura Sichero, Silvaneide Ferreira, Roman Carlos, Victor Costa, Luisa L. Villa |
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| Přispěvatelé: | Universidade Estadual Paulista (Unesp), Ribeirão Preto School of Dentistry, Universidade de São Paulo (USP), Centro Clínico de Cabeza y Cuello |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Male Epstein-Barr Virus Infections Herpesvirus 4 Human severity Squamous Papillomatosis Alphapapillomavirus medicine.disease_cause Gastroenterology Polymerase Chain Reaction Severity of Illness Index law.invention 0302 clinical medicine law Prevalence Medicine Child Respiratory Tract Infections Polymerase chain reaction In Situ Hybridization virus diseases Middle Aged 030220 oncology & carcinogenesis Child Preschool Immunohistochemistry Female Adult medicine.medical_specialty Adolescent Genotype Squamous papillomatosis In situ hybridization Virus 03 medical and health sciences Young Adult human papilloma virus Internal medicine Humans CD21 Epstein-Barr virus Genotyping Aged Retrospective Studies Laryngoscopy business.industry Papillomavirus Infections Infant Epstein–Barr virus 030104 developmental biology Otorhinolaryngology DNA Viral Receptors Complement 3d Recurrent Respiratory Papillomatosis business |
| Zdroj: | Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| Popis: | Made available in DSpace on 2020-12-12T01:51:12Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-01-01 Objective: The objective was to investigate the prevalence of the Epstein-Barr virus (EBV) and its association with human papilloma virus (HPV) detection, clinicopathological features, and the severity of recurrent respiratory papillomatosis (RRP). Methods: Cases of juvenile recurrent respiratory papillomatosis (JRRP) (n = 36) and adult recurrent respiratory papillomatosis (ARRP) (n = 44) were collected retrospectively and subdivided into low- and high-risk severity groups based on the Derkay score. We performed HPV detection and genotyping using a reverse hybridization protocol and investigated the presence of EBV by polymerase chain reaction (PCR) and in situ hybridization. CD21 levels were accessed by immunohistochemistry. Results: All samples were HPV-positive, including 49 cases of HPV 6, 26 cases of HPV 11, four cases of HPV 6 and 11 coinfections, and one case of HPV 16. EBV-DNA was detected in nine samples by PCR, although none of the cases were positive by means of in situ hybridization. CD21 immunoexpression was not statistically associated with any of the variables analyzed. HPV 6 detection was significantly higher in ARRP cases (P = 0.03), whereas HPV 11 was more prevalent in JRRP cases (P = 0.02) and was even more prevalent in JRRP cases of greater severity (Derkay laryngoscopic scale ≥20) (P = 0.04). Conclusion: The presence of EBV does not seem to play an important role in the progression/severity of RRP. Level of Evidence: 4 Laryngoscope, 2019. Department of Biosciences and Oral Diagnosis Institute of Science and Technology São Paulo State University (Unesp), São José dos Campos Department of Stomatology Collective Health and Legal Dentistry Ribeirão Preto School of Dentistry Department of Pathology and Forensic Medicine Ribeirão Preto Medical School University of São Paulo Ribeirão Preto Department of Otolaryngology and Head and Neck Surgery Federal University of Sao Paulo Center for Translational Investigation in Oncology Instituto do Cancer do Estado de Sao Paulo Hospital das Clinicas da Faculdade de Medicina da Universidade de São Paulo Department of Radiology and Oncology Faculdade de Medicina Universidade de São Paulo Centro Clínico de Cabeza y Cuello Department of Biosciences and Oral Diagnosis Institute of Science and Technology São Paulo State University (Unesp), São José dos Campos |
| Databáze: | OpenAIRE |
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