A droplet-chip/mass spectrometry approach to study organic synthesis at nanoliter scale
| Autor: | Kirsten Zeitler, Detlev Belder, Rico Warias, Josef J. Heiland, Stefan Ohla, R. J. Beulig |
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| Rok vydání: | 2017 |
| Předmět: |
Analyte
Electrospray Chromatography Scale (chemistry) 010401 analytical chemistry Microfluidics Biomedical Engineering Bioengineering General Chemistry 010402 general chemistry Mass spectrometry Chip 01 natural sciences Biochemistry 0104 chemical sciences chemistry.chemical_compound chemistry Organic synthesis Body orifice |
| Zdroj: | Lab on a Chip. 17:1996-2002 |
| ISSN: | 1473-0189 1473-0197 |
| Popis: | A droplet-based microfluidic device with seamless hyphenation to electrospray mass spectrometry was developed to rapidly investigate organic reactions in segmented flow providing a versatile tool for drug development. A chip-MS interface with an integrated counterelectrode allowed for a flexible positioning of the chip-emitter in front of the MS orifice as well as an independent adjustment of the electrospray potentials. This was necessary to avoid contamination of the mass spectrometer as well as sample overloading due to the high analyte concentrations. The device was exemplarily applied to study the scope of an amino-catalyzed domino reaction with low picomole amount of catalyst in individual nanoliter sized droplets. |
| Databáze: | OpenAIRE |
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