Constitutive interaction between 4-1BB and 4-1BBL on murine LPS-activated bone marrow dendritic cells masks detection of 4-1BBL by TKS-1 but not 19H3 antibody
| Autor: | Tania H. Watts, Achire N. Mbanwi, Kuan Chung Wang, Gloria H. Y. Lin |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Lipopolysaccharides Genotype Immunology Antigen presentation B-cell receptor Bone Marrow Cells Cell Separation Antibodies 03 medical and health sciences Epitopes Mice Tumor Necrosis Factor Receptor Superfamily Member 9 0302 clinical medicine Antibody Specificity Immunology and Allergy Cytotoxic T cell Animals Antigen-presenting cell Cells Cultured Mice Knockout CD40 biology Follicular dendritic cells ZAP70 Reproducibility of Results Dendritic Cells Flow Cytometry Molecular biology 3. Good health Mice Inbred C57BL 030104 developmental biology 4-1BB Ligand Phenotype biology.protein Interleukin 12 030215 immunology Protein Binding |
| Zdroj: | Journal of immunological methods. 450 |
| ISSN: | 1872-7905 |
| Popis: | 4-1BB is a TNFR family member associated with NF-κB mediated survival signaling. 4-1BB is widely expressed on activated cells of the immune system, including activated T cells, NK cells and dendritic cells. Its ligand, 4-1BBL, is transiently expressed on activated antigen presenting cells and at low levels on activated T cells. Although 4-1BBL-deficient mice clearly demonstrate a role for 4-1BBL in CD8 T cell responses to viruses such as influenza, 4-1BBL can be difficult to detect following infection of mice. Here we provide evidence for a constitutive interaction between endogenous 4-1BB and 4-1BBL on LPS activated bone marrow-derived murine dendritic cells that can mask its detection, with implications for measurement of 4-1BBL expression. The masking of 4-1BBL by its receptor results in loss of reactivity to the anti-4-1BBL antibody TKS-1, whereas the 19H3 antibody binds to 4-1BBL in the presence or absence of 4-1BB. Moreover, 4-1BB/4-1BBL interaction can occur in trans between 4-1BB+/+ and 4-1BB-/- dendritic cells in culture. These data suggest that 19H3 is the preferable antibody to use to detect 4-1BBL in the presence of its receptor. |
| Databáze: | OpenAIRE |
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