Microarray analysis of long non-coding RNA expression profiles uncovers a Toxoplasma-induced negative regulation of host immune signaling
| Autor: | Liya Cai, Wenting Zhang, Liyang Huang, Shaohui Liang, Sheng-Nan Zhang, Yu Zhang, Qimei Wei, Wenquan Liu |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Male UNC93B1 Foreskin Down-Regulation Toxoplasma gondii Microarray lcsh:Infectious and parasitic diseases 03 medical and health sciences 0302 clinical medicine Immune system parasitic diseases Humans lcsh:RC109-216 RNA Messenger KEGG Gene Gene knockdown biology Microarray analysis techniques Sequence Analysis RNA Research Gene Expression Profiling Immune signaling RNA Fibroblasts biology.organism_classification Microarray Analysis Long non-coding RNA Cell biology 030104 developmental biology Infectious Diseases Gene Expression Regulation Host-Pathogen Interactions Cytokines RNA Long Noncoding Parasitology Toxoplasma 030215 immunology Signal Transduction |
| Zdroj: | Parasites & Vectors Parasites & Vectors, Vol 11, Iss 1, Pp 1-13 (2018) |
| ISSN: | 1756-3305 |
| DOI: | 10.1186/s13071-018-2697-8 |
| Popis: | Background Toxoplasma gondii is an obligate intracellular protozoan parasite that can infect mammalian cells and thereby regulate host gene expression. The long non-coding RNAs (lncRNAs) have been demonstrated to be an important class of RNA molecules that regulate many biological processes, including host-pathogen interactions. However, the role of host lncRNAs in the response to T. gondii infection remains largely unknown. Methods We applied a microarray approach to determine the differential expression profiles of both lncRNAs and mRNAs in the human foreskin fibroblast (HFF) cells after T. gondii infection. The Gene Ontology and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were performed to reveal the potential functions of T. gondii-induced genes. Based on the co-expression networks of lncRNAs and immune-related genes, the role of NONSHAT022487 on the regulation of UNC93B1 related immune signaling was investigated by the knockdown and over-expression of lncRNA in human macrophage derived from the PMA-induced promonocytic cell line THP-1. Results Our data showed that 996 lncRNAs and 109 mRNAs in HFF cells were significantly and differentially expressed following T. gondii infection (fold change ≥ 5, P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
| Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |