The VIP-VPAC2 neuropeptidergic axis is a cellular pacemaking hub of the suprachiasmatic nucleus circadian circuit

Autor: Johanna E. Chesham, Nicola J. Smyllie, Marco Brancaccio, Mathew D. Edwards, Ryan Hamnett, Andrew P. Patton, Michael H. Hastings, Elizabeth S. Maywood
Přispěvatelé: Patton, Andrew P. [0000-0003-2666-4254], Smyllie, Nicola J. [0000-0002-6324-1421], Hamnett, Ryan [0000-0002-9118-1585], Chesham, Johanna E. [0000-0002-8981-2667], Brancaccio, Marco [0000-0001-8053-5221], Hastings, Michael H. [0000-0001-8576-6651], Apollo - University of Cambridge Repository, Patton, Andrew P [0000-0003-2666-4254], Smyllie, Nicola J [0000-0002-6324-1421], Chesham, Johanna E [0000-0002-8981-2667], Hastings, Michael H [0000-0001-8576-6651]
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0301 basic medicine
Male
Vasoactive intestinal peptide
Circadian clock
42/109
General Physics and Astronomy
14
Mice
0302 clinical medicine
Genes
Reporter
lcsh:Science
64
Neurons
Multidisciplinary
Suprachiasmatic nucleus
Circadian Rhythm
Receptors
Vasoactive Intestinal Peptide
Type II
Female
Suprachiasmatic Nucleus
631/378
hormones
hormone substitutes
and hormone antagonists
Signal Transduction
Vasoactive Intestinal Peptide
Science
Period (gene)
Neuropeptide
Optogenetics
Biology
Circadian mechanisms
General Biochemistry
Genetics and Molecular Biology
Article
03 medical and health sciences
Circadian Clocks
Oscillometry
Oscillation (cell signaling)
631/378/1385
Animals
Circadian rhythm
42
631/378/1385/1330
Genetic Complementation Test
General Chemistry
64/110
Cryptochromes
Mice
Inbred C57BL
030104 developmental biology
nervous system
14/63
lcsh:Q
sense organs
Circadian rhythms and sleep
Neuroscience
030217 neurology & neurosurgery
Zdroj: Nature Communications
Nature Communications, Vol 11, Iss 1, Pp 1-17 (2020)
ISSN: 2041-1723
Popis: The hypothalamic suprachiasmatic nuclei (SCN) are the principal mammalian circadian timekeeper, co-ordinating organism-wide daily and seasonal rhythms. To achieve this, cell-autonomous circadian timing by the ~20,000 SCN cells is welded into a tight circuit-wide ensemble oscillation. This creates essential, network-level emergent properties of precise, high-amplitude oscillation with tightly defined ensemble period and phase. Although synchronised, regional cell groups exhibit differentially phased activity, creating stereotypical spatiotemporal circadian waves of cellular activation across the circuit. The cellular circuit pacemaking components that generate these critical emergent properties are unknown. Using intersectional genetics and real-time imaging, we show that SCN cells expressing vasoactive intestinal polypeptide (VIP) or its cognate receptor, VPAC2, are neurochemically and electrophysiologically distinct, but together they control de novo rhythmicity, setting ensemble period and phase with circuit-level spatiotemporal complexity. The VIP/VPAC2 cellular axis is therefore a neurochemically and topologically specific pacemaker hub that determines the emergent properties of the SCN timekeeper.
Circadian activity modulation in the suprachiasmatic nucleus (SCN) is a network-level emergent property that requires neuropeptide VIP signaling, yet the precise cellular mechanisms are unknown. Patton et al. show that cells expressing VIP or its receptor VPAC2 together determine these emergent properties of the SCN.
Databáze: OpenAIRE
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