Enhancement of viral RNA-induced interferon production of L cells treated with insulin and amphotericin B methyl ester
| Autor: | David J. Giron, Arnold K. Fowler, Janis A. Hoffman, Katherine C. Smith |
|---|---|
| Rok vydání: | 1981 |
| Předmět: |
Interferon Inducers
medicine.medical_treatment Immunology Cell Balanced salt solution Biology Virus L Cells Interferon Virology Amphotericin B medicine Insulin Encephalomyocarditis virus Dose-Response Relationship Drug RNA Drug Synergism Molecular biology medicine.anatomical_structure Amphotericin B methyl ester Cell culture RNA Viral Interferons medicine.drug |
| Zdroj: | Journal of interferon research. 1(4) |
| ISSN: | 0197-8357 |
| Popis: | A study was initiated to develop a more efficient method of producing high-titered L cell interferon. The induction of interferon by viral infectious RNA could be augmented by pretreating the cell cultures with insulin and infecting in the presence of Amphotericin B methyl ester (AmBME). L cells were treated for 24 hr with different concentrations of insulin and then challenged with MM virus RNA suspended in a balanced salt solution with and without AmBME. The fluids were collected 48 hr after infection and assayed for interferon activity. Interferon production was markedly increased in insulin-treated cultures and that the increase was dose-dependent. |
| Databáze: | OpenAIRE |
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