Phosphocreatine restores high-energy phosphates in ischemic myocardium: implication for off-pump cardiac revascularization
| Autor: | Linda Vona-Davis, Gordon F. Murray, David K. Murray, Ganga Prabhakar, Paresh Lakhani |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Male
medicine.medical_specialty Time Factors Phosphocreatine medicine.medical_treatment Ischemia Drug Evaluation Preclinical Myocardial Ischemia Myocardial Reperfusion Revascularization Phosphates Rats Sprague-Dawley chemistry.chemical_compound Adenosine Triphosphate Internal medicine medicine Animals Glycolysis Phosphorylation Saline Ligation Analysis of Variance business.industry Myocardium Phosphorus Isotopes medicine.disease Coronary Vessels Magnetic Resonance Imaging Rats Disease Models Animal medicine.anatomical_structure Endocrinology chemistry Injections Intravenous Ischemic Preconditioning Myocardial Ischemic preconditioning Surgery business Energy Metabolism Artery |
| Zdroj: | Journal of the American College of Surgeons. 197(5) |
| ISSN: | 1072-7515 |
| Popis: | High-energy phosphate metabolism is altered in the ischemic myocardium. We investigated the effects of in vivo administration of phosphocreatine (PCr) in a transient ischemic rat model to emulate off-pump myocardial revascularization.Rats received either PCr (100 mg/kg) or saline intravenously 1 hour before surgery. Regional ischemia was maintained for 12 minutes by ligation of the left anterior descending artery and compared with sham-operated animals. Cardiac tissue was studied for ATP, PCr, and inorganic phosphate (Pi) using (31)P-cryo-NMR. Results were compared by ANOVA.Levels of ATP were significantly (p0.01) lower in the ischemic hearts compared with controls; Pi and PCr remained unchanged. The PCr/Pi ratio was altered in ischemic hearts, reflecting an increased energy demand. PCr administration significantly (p0.01) elevated the content of PCr and ATP in both normal and ischemic hearts.PCr restores high-energy phosphates and attenuates metabolic stress during periods of myocardial ischemia in the rat. Preconditioning with PCr may serve as a useful adjunct to off-pump coronary revascularization. |
| Databáze: | OpenAIRE |
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