Establishment of nasal tolerance to heat shock protein-60 alleviates atherosclerosis by inducing TGF-β-dependent regulatory T cells
| Autor: | Guiwen Yi, Yanping Ding, Qiutang Zeng, Haiyu Li, Wenkai Yang |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Male
animal structures Regulatory T cell medicine.medical_treatment Biomedical Engineering Mice Transgenic chemical and pharmacologic phenomena Inflammation Biology T-Lymphocytes Regulatory Biochemistry Immune tolerance Biomaterials Mice Transforming Growth Factor beta Heat shock protein Immune Tolerance Genetics medicine Animals IL-2 receptor Administration Intranasal Earth-Surface Processes fungi FOXP3 Chaperonin 60 Atherosclerosis Mice Inbred C57BL Treatment Outcome Cytokine medicine.anatomical_structure Immunology Nasal administration medicine.symptom |
| Zdroj: | Journal of Huazhong University of Science and Technology [Medical Sciences]. 32:24-30 |
| ISSN: | 1993-1352 1672-0733 |
| DOI: | 10.1007/s11596-012-0004-z |
| Popis: | Mounting evidence supports that a newly identified regulatory T cell (Treg), CD4(+)LAP(+) Treg, is associated with oral tolerance induction and following inhibition of atherosclerosis, but little is described about whether nasal tolerance to antigen likewise induces the novel Tregs production and the relevant antiatherosclerotic benefit. We investigated the effect of nasal administration of heat shock protein-60 (HSP60) on atherogenesis. HSP60 or phosphate buffer solution (PBS) was nasally administered to six-week-old male ApoE(-/-) mice. At the 10th week after the nasal administration, there was a significant decrease in atherosclerotic plaque areas of aortic roots in the HSP60-treated mice as compared with those in the PBS-treated mice. Atherosclerosis suppression was accompanied with a significant increase in CD4(+)LAP(+) and CD4(+)CD25(+)Foxp3(+) Tregs and a concurrently increased production of TGF-β in the HSP60-treated mice. The protective effect of HSP60 was offset by injection of anti-TGF-β antibody. It is concluded that nasal administration of HSP60 can inhibit atherosclerotic formation through immune tolerance which is established by Tregs depending on the induction of anti-inflammatory cytokine TGF-β. Immune tolerance induced by nasal administration of HSP60 may provide an alternative therapeutic method for atherosclerosis. |
| Databáze: | OpenAIRE |
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