The pleiotropic molecule NGF regulates the in vitro properties of fibroblasts, keratinocytes, and endothelial cells: implications for wound healing
| Autor: | Luigi Aloe, Luciana Giardino, Natalia Gostynska, Micaela Pannella, Laura Calzà, Maria Luisa Rocco |
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| Přispěvatelé: | Gostynska N., Pannella M., Rocco M.L., Giardino L., Aloe L., Calza' L. |
| Rok vydání: | 2020 |
| Předmět: |
Keratinocytes
Male 0301 basic medicine Cell type Physiology Angiogenesis Human Umbilical Vein Endothelial Cell Cell Line Mice 03 medical and health sciences 0302 clinical medicine Cell Movement Nerve Growth Factor Human Umbilical Vein Endothelial Cells medicine Animals Humans Cell migration Fibroblast Cell Proliferation Skin NGF Endothelial Cell Mice Inbred ICR Wound Healing Animal Chemistry Endothelial Cells Cell Biology Fibroblasts In vitro Pathophysiology Cell biology Angiogenesi 030104 developmental biology medicine.anatomical_structure Nerve growth factor 030220 oncology & carcinogenesis Wound healing Keratinocyte Human |
| Zdroj: | American Journal of Physiology-Cell Physiology. 318:C360-C371 |
| ISSN: | 1522-1563 0363-6143 |
| DOI: | 10.1152/ajpcell.00180.2019 |
| Popis: | Nerve growth factor (NGF) is recognized as a pleiotropic molecule, exerting a variety of biological effects on different cell types and pathophysiological conditions, and its role in tissue wound healing has been recently highlighted. However, the preferential cellular target of NGF is still elusive in the complex cellular and molecular cross talk that accompanies wound healing. Thus, to explore possible NGF cellular targets in skin wound healing, we investigated the in vitro NGF responsiveness of keratinocytes (cell line HEKa), fibroblasts (cell line BJ), and endothelial cells (cell line HUVEC), also in the presence of adverse microenvironmental conditions, e.g., hyperglycemia. The main results are summarized as follows: 1) NGF stimulates keratinocyte proliferation and HUVEC proliferation and angiogenesis in a dose-dependent manner although it has no effect on fibroblast proliferation; 2) NGF stimulates keratinocyte but not fibroblast migration in the wound healing assay; and 3) NGF completely reverts the proliferation impairment of keratinocytes and the angiogenesis impairment of HUVECs induced by high d-glucose concentration in the culture medium. These results contribute to better understanding possible targets for the therapeutic use of NGF in skin repair. |
| Databáze: | OpenAIRE |
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