Biosynthesis of beta-sitosterol and stigmasterol in Croton sublyratus proceeds via a mixed origin of isoprene units
| Autor: | Wanchai De-Eknamkul, Buppachart Potduang |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Magnetic Resonance Spectroscopy
Time Factors Stereochemistry Stigmasterol Mevalonic Acid Plant Science Horticulture Biology Biochemistry chemistry.chemical_compound Tissue culture Xylulose Hemiterpenes Biosynthesis Pentanes Butadienes Molecular Biology Isoprene Carbon Isotopes Phytosterol General Medicine Sitosterols Plant Leaves Metabolic pathway Glucose chemistry Callus Mevalonate pathway Chromatography Thin Layer Croton |
| Zdroj: | Phytochemistry. 62(3) |
| ISSN: | 0031-9422 |
| Popis: | A green callus culture of Croton sublyratus Kurz established from the leaf explants appeared to actively synthesize two well-known phytosterols, β-sitosterol and stigmasterol. The phytosterol biosynthesis was highly active during the linear phase of the culture. Feeding of [1- 13 C]glucose into the callus culture at this growth phase showed that the label from glucose was highly incorporated into both phytosterols. Isolation of the labeled products followed by 13 C NMR analysis revealed that the phytosterols had their 13 C–labeling patterns consistent with the acquisition of isoprene units via both the mevalonate pathway and the deoxyxylulose pathway with relatively equal contribution. Since the biosynthesis of phytosterol has so far been reported to be mainly from the classical mevalonate pathway, this study provides a new evidence on the biosynthesis of phytosterols via the novel deoxyxylulose pathway. |
| Databáze: | OpenAIRE |
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