Kinetics of the inhibition of human leucocyte elastase by eglin from the leech Hirudo medicinalis
| Autor: | U Seemüller, Antonio Baici |
|---|---|
| Rok vydání: | 1984 |
| Předmět: |
biology
Pancreatic Elastase Stereochemistry Chemistry Macromolecular Substances Elastase Kinetics Leech Proteins Cell Biology biology.organism_classification Leucocyte elastase Biochemistry Hirudo medicinalis Models Chemical Biological significance Leeches Leukocytes Animals Humans Molecular Biology Serpins Research Article |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 0264-6021 |
| Popis: | The rate constants for the inhibition of human leucocyte elastase by eglin from the leech Hirudo medicinalis were determined by using a pre-steady-state kinetic approach. kon and koff for complex-formation and dissociation were 1 × 10(6)M-1 X S-1 and 8 × 10(-4)S-1 respectively. Ki was calculated as the ratio koff/kon = 8 × 10(-10)M, the binding of eglin to elastase was reversible and the inhibition mechanism was of the fully competitive type. The mechanistic properties of the system and the biological significance of the rate constants are discussed. |
| Databáze: | OpenAIRE |
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