Stage-specific responses following infection with Theileria annulata as evaluated using ELISA
| Autor: | C. G. D. Brown, L. Bell-Sakyi, P.F. Rae, Roger L. Spooner, M. Kachani |
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| Rok vydání: | 1992 |
| Předmět: |
Antigenicity
Antibodies Protozoan Fluorescent Antibody Technique Antigens Protozoan Enzyme-Linked Immunosorbent Assay Immune system Antigen Species Specificity Predictive Value of Tests parasitic diseases Animals Direct fluorescent antibody General Veterinary biology General Medicine biology.organism_classification Virology Theileria annulata Theileriasis Infectious Diseases Immunization Insect Science Humoral immunity biology.protein Protozoa Parasitology Cattle Antibody |
| Zdroj: | Parasitology research. 78(1) |
| ISSN: | 0932-0113 |
| Popis: | An enzyme-linked immunosorbent assay (ELISA) using sporozoite, schizont and piroplasm antigens was developed to study the immune response of animals that had been immunised with eitherTheileria annulata sporozoites or schizont-infected cells and then challenged with sporozoites. The aim was to identify the most suitable antigen for a routine screening test and to compare the sensitivity of the latter with that of the indirect fluorescent antibody test (IFAT). As determined by ELISA, cattle produced antibodies to all three antigens, regardless of the method of immunisation. The schizont antigen was the least sensitive, whereas the sporozoite antigen displayed high pre-inoculation values. In contrast, the piroplasm antigen exhibited low non-specific pre-infection levels and high post-immunisation and post-challenge values according to both ELISA and IFAT. Therefore, the latter was thought to be the most appropriate antigen for use in ELISA. |
| Databáze: | OpenAIRE |
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