Colon and liver tissue damage detection using methylated SESN3 and PTK2B genes in circulating cell-free DNA in patients with acute graft-versus-host disease
| Autor: | Florian Scherer, Max Deuter, Nikolas von Bubnoff, Sandra Pennisi, Jesus Duque-Afonso, Miguel Waterhouse, Hartmut Bertz, Jürgen Finke, Dietmar Pfeifer, Justus Duyster, Claudia Wehr, Tim Strüssmann |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
medicine.medical_specialty
Disease Gastroenterology Acute myeloid leukaemia 03 medical and health sciences 0302 clinical medicine Technical Report immune system diseases Internal medicine hemic and lymphatic diseases Biopsy medicine Gene Transplantation PTK2B medicine.diagnostic_test integumentary system business.industry Area under the curve Hematology Methylation Translational research Circulating Cell-Free DNA surgical procedures operative 030220 oncology & carcinogenesis business 030215 immunology |
| Zdroj: | Bone Marrow Transplantation |
| ISSN: | 1476-5365 0268-3369 |
| Popis: | Cell-free DNA (cfDNA) has been investigated in acute graft-versus-host disease (aGvHD) following allogeneic cell transplantation (HSCT). Identifying the tissue of origin of cfDNA in patients with aGvHD is relevant particularly when a biopsy is not feasible. We investigate the cfDNA tissue of origin in patients with aGvHD using methylated gene biomarkers. Patients with liver, colon, or skin aGvHD (n = 28) were analyzed. Liver- and colon-derived cfDNA was measured using a colon- (SESN3) and liver (PTK2B)-specific methylation marker with digital droplet PCR. A statistically significant difference (p p p |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink