Isolation and characterization of novel lipase gene LipHim1 from the DNA isolated from soil samples
| Autor: | Pavan Kumar Pindi, R Raja Srinath, Theetha L. Pavankumar |
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| Rok vydání: | 2013 |
| Předmět: |
Base pair
India Polysorbates Biology Applied Microbiology and Biotechnology Microbiology Homology (biology) chemistry.chemical_compound Open Reading Frames Lipase Gene Soil Microbiology chemistry.chemical_classification Sequence Homology Amino Acid Hydrolysis Fatty Acids Fatty acid General Medicine DNA Amino acid Aeromonas hydrophila Molecular Weight Open reading frame chemistry Biochemistry biology.protein Metagenomics |
| Zdroj: | Journal of microbiology (Seoul, Korea). 52(5) |
| ISSN: | 1976-3794 |
| Popis: | Metagenomics is a magnificent tool to isolate genes from unknown/uncharacterized species and also from organisms that cannot be cultured. In this study, we constructed a metagenomic library from isolated DNA of soil samples collected from Palamuru University campus premises, in Mahabubnagar district of Andhra Pradesh, India. We isolated a novel lipase gene LipHim1, which has an open reading frame of 591 base pairs and encodes ∼23 kDa protein consisting of 196 amino acids. The Lipase LipHim1 showed maximum 32% homology at the protein level with the extracellular Aeromonas hydrophila lipase (Class II, GDSL family) and was significantly different from all other known lipases. The isolated lipase catalyzed the hydrolysis of fatty acid esters of polyoxyethylene sorbitan such as Tween 60. Our results indicate that the isolated lipase gene is novel. |
| Databáze: | OpenAIRE |
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