Cloning and sequencing a putative pyrophosphate-dependent phosphofructokinase gene from Entamoeba histolytica
| Autor: | Min Huang, Kwang-Poo Chang, Richard A. Albach, Rakesh L. Tripathi, Robert G. Kemp |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Molecular Sequence Data
Biophysics Biochemistry Entamoeba histolytica Structural Biology parasitic diseases Genetics Animals Genomic library Amino Acid Sequence Cloning Molecular Gene Peptide sequence Cloning biology Base Sequence Propionibacterium freudenreichii Phosphotransferases food and beverages biology.organism_classification Open reading frame Sequence Alignment Phosphofructokinase |
| Zdroj: | Biochimica et biophysica acta. 1260(2) |
| ISSN: | 0006-3002 |
| Popis: | Pyrophosphate-dependent phosphofructokinase (PPi-PFK) gene from Entamoeba histolytica was cloned from its genomic library and sequenced. The open reading frame has 1149 by and codes for a protein of 41.5 kDa. The deduced amino acid sequence of E. histolytica PPi-PFK has 25 to 28% identity to the PPi-PFKs from Propionibacterium freudenreichii, Naegleria fowleri and potato. The amino acid residues known to contribute to the active site of PPi-PFK from P. freudenreichii are conserved. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|