An improved radioimmunoassay method for the detection of IgG antibodies against cytomegalovirus
| Autor: | Neil Berry, J.E. Grundy, Paul D. Griffiths |
|---|---|
| Rok vydání: | 1986 |
| Předmět: |
medicine.drug_class
Immune Sera Radioimmunoassay Congenital cytomegalovirus infection Antibodies Monoclonal Cytomegalovirus Biology Antibodies Viral Monoclonal antibody medicine.disease_cause medicine.disease Virology Molecular biology Herpesviridae Virus Polyclonal antibodies Immunoglobulin G medicine biology.protein Humans Murine monoclonal antibody Antibody |
| Zdroj: | Journal of Virological Methods. 13:343-350 |
| ISSN: | 0166-0934 |
| DOI: | 10.1016/0166-0934(86)90059-5 |
| Popis: | The non-specific binding seen with human sera in a radioimmunoassay for the detection of IgG antibodies specific for CMV can be reduced greatly by using a murine monoclonal antibody as a radiolabelled detecting antibody. Such non-specific binding formerly obtained with a polyclonal detecting antibody was due to the binding of the polyclonal reagent to factors on the solid phase other than IgG molecules. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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