Bacterial Cell Cultures in a Lab-on-a-Disc: A Simple and Versatile Tool for Quantification of Antibiotic Treatment Efficacy
| Autor: | Anja Boisen, Janus A. J. Haagensen, Mads Peter Soerensen, Claus Sternberg, Søren Molin, Laura Serioli, Trygvi Z. Laksafoss, Kinga Zor |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Microscopy
Confocal Pseudomonas aeruginosa Chemistry medicine.drug_class 010401 analytical chemistry Antibiotics Biofilm 010402 general chemistry medicine.disease_cause 01 natural sciences Bacterial cell structure Treatment efficacy Anti-Bacterial Agents 0104 chemical sciences Analytical Chemistry Drug development Batch Cell Culture Techniques Cell culture Biofilms Lab-On-A-Chip Devices medicine Biomass Lab on a disc Biomedical engineering |
| Zdroj: | Serioli, L, Laksafoss, T Z, Haagensen, J A J, Sternberg, C, Sørensen, M P, Molin, S, Zor, K & Boisen, A 2020, ' Bacterial Cell Cultures in a Lab-on-a-Disc: A Simple and Versatile Tool for Quantification of Antibiotic Treatment Efficacy ', Analytical Chemistry, vol. 92, no. 20, pp. 13871–13879 . https://doi.org/10.1021/acs.analchem.0c02582 |
| Popis: | Pathogenic bacterial biofilms can be life-threatening, greatly decrease patient's quality of life, and are a substantial burden on the healthcare system. Current methods for evaluation of antibacterial treatments in clinics and in vitro systems used in drug development and screening either do not facilitate biofilm formation or are cumbersome to operate, need large reagent volumes, and are costly, limiting their usability. To address these issues, this work presents the development of a robust in vitro cell culture platform compatible with confocal microscopy. The platform shaped as a compact disc facilitates long-term bacterial culture without external pumps and tubing and can be operated for several days without additional liquid handling. As an example, Pseudomonas aeruginosa biofilm is grown from single cells, and it is shown that (1) the platform delivers reproducible and reliable results; (2) growth is dependent on flow rate and growth medium composition; and (3) efficacy of antibiotic treatment depends on the formed biofilm. This platform enables biofilm growth, quantification, and treatment as in a conventional flow setup while decreasing the application barrier of lab-on-chip systems. It provides an easy-to-use, affordable option for end users working with cell culturing in relation to, e.g., diagnostics and drug screening. |
| Databáze: | OpenAIRE |
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