β-Galactosidase immunoassay for the measurement of cyclic AMP
| Autor: | Evelyne Joseph, Jean-Luc Guesdon |
|---|---|
| Rok vydání: | 1982 |
| Předmět: |
Genotype
Biophysics Immunoglobulins Adenylate kinase medicine.disease_cause Biochemistry Cyclase chemistry.chemical_compound Cyclic AMP Escherichia coli medicine Molecular Biology Edetic Acid Immunoassay chemistry.chemical_classification Chromatography medicine.diagnostic_test Radioimmunoassay Cell Biology beta-Galactosidase Galactosidases Enzyme chemistry Mutation Derivative (chemistry) Adenylyl Cyclases Conjugate |
| Zdroj: | Analytical Biochemistry. 119:335-340 |
| ISSN: | 0003-2697 |
| DOI: | 10.1016/0003-2697(82)90595-4 |
| Popis: | A specific and sensitive β-galactosidase immunoassay is described. A cyclic AMP-β-galactosidase conjugate is used as the labeled cyclic AMP derivative in a competitive binding assay. By determining the β-galactosidase activity, the concentration of the antibody-bound conjugate is easily detected. Cyclic AMP levels and adenylate cyclase activities in Escherichia coli K12 can be determined by using this β-galactosidase immunoassay. Comparative studies performed between this enzyme immunoassay and the conventional radioimmunoassay show satisfactory correlation between the two methods. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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