Rho-associated kinase inhibitor Y-27632 promotes survival of cynomolgus monkey embryonic stem cells
| Autor: | Makoto Takenoshita, Toshiyuki Takehara, Kanji Fukuda, Yuta Onodera, Yoshihiko Hosoi, Takeshi Teramura, Naoto Fukunaga, Norimasa Sagawa, Ryo Kakegawa |
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| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: |
KOSR
Embryology Cell Survival Pyridines Cellular differentiation Biology primate Cell Line Mice Neurosphere Genetics Animals Molecular Biology Protein Kinase Inhibitors Cryopreservation Induced stem cells rho-Associated Kinases Obstetrics and Gynecology Cell Differentiation Cell Biology Articles differentiation embryonic stem cells pluripotency Amides Neural stem cell Cell biology Macaca fascicularis P19 cell Reproductive Medicine Stem cell Developmental Biology Adult stem cell Stem Cell Transplantation |
| Zdroj: | Molecular Human Reproduction |
| ISSN: | 1460-2407 1360-9947 |
| Popis: | Non-human primates are suitable models for preclinical research aimed at cell-replacement therapies. Recently, it has been reported that Rho-associated kinase inhibitor Y-27632 markedly reduced dissociation-induced apoptosis of human embryonic stem (hES) cells, and is expected as a novel supplement for hES cell maintenance or differentiation inductions; however, the effects of the chemical are still to be determined in model animals. Here, we demonstrated the effect of Y-27632 on cynomolgus monkey ES (cyES) cells. Also, in cyES cells, Y-27632 treatment dramatically improved the efficiency of colony formation from single cells without affecting the pluripotent state and karyotype. Y-27632 supplementation was also effective for feeder-free culture and differentiation induction. Neural stem cells directly induced from cyES cells could give rise to neurons, astrocytes and dopamine producing cells. The present result not only suggests that the chemical was effective for improving the culture system of primate ES cells, but also the similarity between cyES and hES cells regarding the reactions to the chemical, which might be further evidence that cyES cells are superior models for hES cells. |
| Databáze: | OpenAIRE |
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