Assessment of Minimal Residual Disease in Childhood Acute Lymphoblastic Leukemia: A Multicenter Study From Turkey
Autor: | Cinar, Suzan, Yılmaz, Şebnem, Koçak, Ülker, Baytan, Birol, Celkan, Tiraje, Meral Güneş, Adalet, Yılmaz Karapınar, Deniz, Kaya, Zuhre, Baysal, Birsen, Tüfekçi, Özlem, Sezgin Evim, Melike, Ören, Hale |
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Jazyk: | angličtina |
Rok vydání: | 2022 |
Předmět: |
Risk
Prognostic-Factors Neoplasm Residual Trial Aieop-Bfm Turkey Gene Rearrangements acute lymphoblastic leukemia Adolescent Patients Disease-Free Survival hemic and lymphatic diseases Humans Child Children childhood Retrospective Studies Experience Hematology Precursor Cell Lymphoblastic Leukemia-Lymphoma T-Cell Prognosis body regions Oncology Pediatrics Perinatology and Child Health minimal residual disease Therapy Consecutive Trials |
Popis: | Assestment of minimal residual disease (MRD) in childhood acute lymphoblastic leukemia (ALL) is of utmost importance both for risk classification and tailoring of the therapy. The data of pediatric ALL patients that received treatment with Berlin-Frankfurt-Munster (BFM) protocols were retrospectively collected from 5 university hospitals in Turkey. Of the 1388 patients enrolled in the study 390 were treated according to MRD-based protocols. MRD assestment was with real time quantitative polymerase chain reaction (qPCR) in 283 patients and with multiparametric flow cytometry (MFC)-MRD in 107 patients. MRD monitoring had upstaged a total of 8 patients (2%) from intermediate risk group to high-risk group. Univariate analysis revealed age 10 years or above, prednisone poor response, PCR-MRD >= 10(-3) on day 33 and on day 78 as poor prognostic factors affecting event-free survival (EFS). Detection of >10% blasts on day 15 with MFC (MFC-high-risk group) was not shown to affect EFS and/or overall survival (log-rank P=0.339). Multiple logistic regression analysis revealed PCR-MRD >= 10(-3) on day 78 as the only poor prognostic factor affecting EFS (odds ratio: 8.03; 95% confidence interval: 2.5-25; P=0.000). It is very important to establish the infrastructure and ensure necessary standardization for both MRD methods for optimal management of children with ALL. |
Databáze: | OpenAIRE |
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