A novel plant glutathione peroxidase-like protein provides tolerance to oxygen radicals generated by paraquat inEscherichia coli
| Autor: | Doron Holland, Yuval Eshdat, Zehava Faltin, Avi Perl, Gozal Ben-Hayyim |
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| Rok vydání: | 1994 |
| Předmět: |
Paraquat
Citrus Free Radicals Biophysics Gene Expression medicine.disease_cause Biochemistry chemistry.chemical_compound Superoxides Structural Biology Escherichia coli Genetics medicine Salt tolerance Molecular Biology chemistry.chemical_classification Glutathione Peroxidase biology Superoxide Dismutase Glutathione peroxidase Drug Tolerance Cell Biology biology.organism_classification Enterobacteriaceae Enzyme chemistry Oxidative stress biology.protein Transformation Bacterial Bacteria Plasmids Peroxidase |
| Zdroj: | FEBS Letters. 337:52-55 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/0014-5793(94)80628-4 |
| Popis: | Citrus salt-stress associated protein (Cit-SAP) reveals significant sequence homology to mammalian glutathione peroxidase (GP). In an attempt to assign biological function to this protein, transformed E. coli cells expressing Cit-SAP were examined for their ability to tolerate free radicals formed by paraquat, an O−2 radical forming agent. In the presence of paraquat, the survival rate of the transformed bacteria expressing Cit-SAP was much higher as compared to the wild-type bacteria. The results support the assumption that Cit-SAP is a plant GP-like protein which participate in the enzymatic system aimed at scavenging oxygen free-radicals in plants. |
| Databáze: | OpenAIRE |
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