Matrix-assisted laser desorption/ionization mass spectrometry peptide sequencing utilizing selective N-terminal bromoacetylation
| Autor: | Hie-Joon Kim, Jin-Su Song |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Molecular Sequence Data
Lysine Biophysics Mass spectrometry Biochemistry Ion Hemoglobins Sequence Analysis Protein Animals Amino Acid Sequence Tyrosine Molecular Biology Chromatography Tandem Chemistry Acetylation Serum Albumin Bovine Cell Biology Hydrogen-Ion Concentration Bromine Combinatorial chemistry Matrix-assisted laser desorption/ionization Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Mass spectrum Cattle Peptides |
| Zdroj: | Analytical Biochemistry. 423:269-276 |
| ISSN: | 0003-2697 |
| Popis: | In tandem mass spectrometric peptide sequencing, simplifying the mass spectrum is often desirable. The b-series ions were distinguished from the y-series ions in the MALDI TOF-TOF spectra by incorporating a bromine-tag to the N-terminal amino group through rapid and selective acetylation using bromoacetic anhydride without blocking the lysine and tyrosine residues. The 51:49 ratio of Br-79 and Br-81 isotopes facilitated identification of ions carrying the tag. With the Br-tag in the b-series ions, N-terminal sequencing of tryptic peptides from hemoglobin as well as model peptides was straightforward. When the b-ions were low in intensity, ions without the Br-tag were identified as y-ions and used for sequencing. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect