Studies of immunoreactivity to human lacrimal gland fractions in patients with ophthalmic Graves’ disease
| Autor: | Shih-Lieh Fang, S. H. Ingbar, A. Trewin, Lewis E. Braverman, J. R. Wall |
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| Rok vydání: | 1978 |
| Předmět: |
Adult
Male Pathology medicine.medical_specialty Endocrinology Diabetes and Metabolism Graves' disease Eye disease Fluorescent Antibody Technique Lacrimal gland In Vitro Techniques Lymphocyte Activation Antibodies Antigen-Antibody Reactions Pathogenesis Endocrinology Antigen medicine Humans Macrophage Migration-Inhibitory Factors Aged biology business.industry Lacrimal Apparatus Hemagglutination Tests Middle Aged medicine.disease Graves Disease Lymphatic system medicine.anatomical_structure Peripheral blood lymphocyte biology.protein Female Antibody business Subcellular Fractions |
| Zdroj: | Journal of Endocrinological Investigation. 1:253-258 |
| ISSN: | 1720-8386 0391-4097 |
| DOI: | 10.1007/bf03350389 |
| Popis: | In vitro evidence for immunoreactivity against human lacrimal gland fractions was sought in patients with ophthalmic Graves' disease. There was no significant increase in interstitial lymphoid tissue on lacrimal gland biopsy. Serum antibodies against lacrimal fractions were not detected using the indirect immunofluorescent technique. Using the tanned cell hemagglutination test with lacrimal antigen, antibodies were detected in 2 of 15 patients with eye disease, 2 of 15 hyperthyroid patients without eye disease, and 2 of 20 normal subjects. Macrophage inhibitory factor (MIF) production in response to human lacrimal extract was not demonstrated in any of 11 patients with eye disease tested. On the other hand, MIF was demonstrated in 2 of 10 patients with Graves' disease selected for absence of eye disease. Significant peripheral blood lymphocyte transformation in response to human lacrimal extract, or a soluble or membrane fraction, was demonstrated in 9 of 22 patients tested; in 6 of 21 patients to extract, in 3 of 21 patients to a soluble fraction and in 7 of 10 patients to a membrane fraction. The possible significance of lacrimal gland inflammation and role(s) in the pathogenesis of ophthalmic Graves' disease are discussed. |
| Databáze: | OpenAIRE |
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