lncRNA HOTAIR knockdown suppresses gastric cancer cell biological activities
| Autor: | Ma Yong, Zhu Yu, Zhou Jin, Feng Yongheng, Pan Chao, Yang Kun-xing, Yang Shi-yong |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
lcsh:TX341-641 CCND2 Flow cytometry CCND1 03 medical and health sciences 0302 clinical medicine Cyclin D1 HOTAIR medicine MTT assay Viability assay miRNA‐206 Original Research Gene knockdown medicine.diagnostic_test Chemistry gastric cancer Cancer medicine.disease 030104 developmental biology Apoptosis 030220 oncology & carcinogenesis Cancer research lcsh:Nutrition. Foods and food supply Food Science |
| Zdroj: | Food Science & Nutrition Food Science & Nutrition, 9(1):123-134 Food Science & Nutrition, Vol 9, Iss 1, Pp 123-134 (2021) |
| ISSN: | 2048-7177 |
| Popis: | The aim of this study was to characterize the involvement of long noncoding RNA HOTAIR in gastric cancer development. Measurement of HOTAIR and miRNA‐206 expression by in situ hybridization (ISH) and analyzed for the correlation between HOTAIR and miRNA‐206 in gastric cancer tissues. To evaluate the effects of HOTAIR in gastric cancer, MTT assay, flow cytometry, transwell, and wound healing assays were applied. To explain the mechanism behind HOTAIR's involvement, the expression of proteins related to it was also measured by Western blotting. Finally, correlations among related factors were determined by a luciferase target experiment. HOTAIR expression significantly increased, and miRNA‐206 expression significantly decreased in cancer tissues (p HOTAIR knockdown had antitumor effects by suppressing CCND1 and CCND2 expression by stimulating miRNA‐206 in gastric cancer in vitro study. |
| Databáze: | OpenAIRE |
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