In-vitro circadian rhythm of murine bone marrow progenitor production
| Autor: | Jacques Beau, Francis Lévi, Arnaud F. Ledain, Philippe Bourin, Dominique Mille |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Male
Physiology Ratón Circadian clock In Vitro Techniques Biology Colony-Forming Units Assay Mice In vivo Physiology (medical) medicine Animals Humans Circadian rhythm Progenitor cell Chronotherapy Mice Inbred BALB C Granulocyte-Macrophage Colony-Stimulating Factor Hematopoietic Stem Cells Recombinant Proteins In vitro Circadian Rhythm Hematopoiesis Cell biology Haematopoiesis medicine.anatomical_structure Immunology Bone marrow |
| Zdroj: | Chronobiology International. 19:57-67 |
| ISSN: | 1525-6073 0742-0528 |
| DOI: | 10.1081/cbi-120002677 |
| Popis: | Hematopoietic processes display 24h rhythms both in rodents and in human beings. We hypothesized these rhythms to be in part generated by a circadian oscillator within the bone marrow. The ability of murine bone marrow granulo-monocytic (GM) precursors to form colonies following colony-stimulating factor (rm GM-CSF) exposure was investigated in liquid culture samples obtained every 3 h for a span of up to 198 h. The CFU-GM count varied rhythmically over the first 4 d of culture, with a reproducible maximum in the early morning hours, similar to that observed in vivo. These experiments provide the first evidence that bone marrow progenitors sustain in vitro circadian rhythmicity, and they demonstrate the presence of a circadian time-keeping system within these cells. The results support the potential usefulness of bone marrow cultures for investigating chronopharmacologic effects of anticancer drugs and cytokines on this target system. |
| Databáze: | OpenAIRE |
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