PLGA microspheres phagocytosis by pig alveolar macrophages: influence of poly(vinyl alcohol) concentration, nature of loaded-protein and copolymer nature
| Autor: | Le Verge R, Anne-Marie Torché, Jestin A, Le Corre P, Emmanuel Albina |
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| Rok vydání: | 2000 |
| Předmět: |
Vinyl alcohol
Polymers Swine Phagocytosis Serum albumin Pharmaceutical Science Immunoglobulins chemistry.chemical_compound Polylactic Acid-Polyglycolic Acid Copolymer Macrophages Alveolar medicine Animals Lactic Acid Bovine serum albumin biology Serum Albumin Bovine Flow Cytometry Microspheres medicine.anatomical_structure chemistry Biochemistry Polyvinyl Alcohol Alveolar macrophage biology.protein Biophysics Immunoglobulin Y Pulmonary alveolus Drug carrier Polyglycolic Acid |
| Zdroj: | Journal of drug targeting. 7(5) |
| ISSN: | 1061-186X |
| Popis: | The study aimed to investigate on a pig alveolar macrophage culture model the influence of: (1) poly(D,L-lactide-co-glycolide) characteristics, (2) the residual poly(vinyl alcohol) (PVA) and (3) the nature of encapsulated proteins, immunoglobulin Y (IgY) or bovine serum albumin, on the microspheres phagocytosis efficiency. The phagocytosis evaluation was performed by flow cytometry and has allowed a screening of microspheres formulations. The hydrophilicity of microspheres resulting from the nature of the polymer and/ or from the residual hydrophilic surface agent (PVA) led to a decrease of phagocytosis intensity. The phagocytosis results of IgY-loaded microspheres strongly suggested that the phagocytosis was increased when the phagocytic cell possessed a receptor for this protein on its surface. |
| Databáze: | OpenAIRE |
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