Atomic force microscopy fishing and mass spectrometry identification of gp120 on immobilized aptamers
| Autor: | Tatyana I Pavlova, Yuri D. Ivanov, Tatyana O. Pleshakova, Sergey P. Radko, Sergei A. Moshkovskii, Alexander I. Archakov, Victor G. Zgoda, P. A. Frantsuzov, Elena Yu Andreeva, Vadim S. Ziborov, N. S. Bukharina, Alexander A. Izotov, Anna L. Kaysheva |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Analyte
Aptamer viruses gp120 HIV-1 envelope glycoprotein Biophysics Human immunodeficiency virus (HIV) Immobilized Nucleic Acids Pharmaceutical Science Molecular Probe Techniques Bioengineering Nanotechnology HIV Envelope Protein gp120 Mass spectrometry medicine.disease_cause Microscopy Atomic Force Mass Spectrometry Biomaterials International Journal of Nanomedicine Drug Discovery medicine Original Research atomic force microscopy Chemistry Atomic force microscopy Organic Chemistry technology industry and agriculture aptamer General Medicine Aptamers Nucleotide Image contrast Target protein |
| Zdroj: | International Journal of Nanomedicine |
| ISSN: | 1178-2013 1176-9114 |
| Popis: | Yuri D Ivanov,1 Natalia S Bukharina,1 Tatyana O Pleshakova,1 Pavel A Frantsuzov,1 Elena Yu Andreeva,1 Anna L Kaysheva,1,2 Victor G Zgoda,1 Alexander A Izotov,1 Tatyana I Pavlova,1 Vadim S Ziborov,1 Sergey P Radko,1 Sergei A Moshkovskii,1 Alexander I Archakov1 1Department of Personalized Medicine, Orekhovich Institute of Biomedical Chemistry of the Russian Academy of Medical Sciences, Moscow, Russia; 2PostgenTech Ltd., Moscow, Russia Abstract: Atomic force microscopy (AFM) was applied to carry out direct and label-free detection of gp120 human immunodeficiency virus type 1 envelope glycoprotein as a target protein. This approach was based on the AFM fishing of gp120 from the analyte solution using anti-gp120 aptamers immobilized on the AFM chip to count gp120/aptamer complexes that were formed on the chip surface. The comparison of image contrasts of fished gp120 against the background of immobilized aptamers and anti-gp120 antibodies on the AFM images was conducted. It was shown that an image contrast of the protein/aptamer complexes was two-fold higher than the contrast of the protein/antibody complexes. Mass spectrometry identification provided an additional confirmation of the target protein presence on the AFM chips after biospecific fishing to avoid any artifacts. Keywords: gp120 HIV-1 envelope glycoprotein, aptamer, atomic force microscopy, mass spectrometry |
| Databáze: | OpenAIRE |
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