Arabidopsis RETICULON-LIKE3 (RTNLB3) and RTNLB8 Participate in Agrobacterium-Mediated Plant Transformation

Autor: Yin-Tzu Liu, Fan-Chen Huang, Bi-Ju Fu, Pei-Ru Chien, Shin-Fei Chi, Hau-Hsuan Hwang, Si-Chi Huang, Yao-Ren Chang
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0106 biological sciences
0301 basic medicine
Transfer DNA
RTNLB
Arabidopsis
Gene Expression
Agrobacterium
01 natural sciences
lcsh:Chemistry
Ti plasmid
Gene Expression Regulation
Plant
Yeasts
Pseudomonas syringae
lcsh:QH301-705.5
Spectroscopy
food and beverages
General Medicine
Agrobacterium tumefaciens
Plants
Genetically Modified
Computer Science Applications
Cell biology
Organ Specificity
Host-Pathogen Interactions
Disease Susceptibility
Protein Binding
DNA
Bacterial
Recombinant Fusion Proteins
Biology
Catalysis
Article
Inorganic Chemistry
03 medical and health sciences
Transformation
Genetic
Physical and Theoretical Chemistry
Molecular Biology
Plant Diseases
Arabidopsis Proteins
Organic Chemistry
fungi
Membrane Proteins
Correction
biology.organism_classification
Transformation (genetics)
030104 developmental biology
lcsh:Biology (General)
lcsh:QD1-999
Mutation
010606 plant biology & botany
Transformation efficiency
Zdroj: International Journal of Molecular Sciences
International Journal of Molecular Sciences, Vol 19, Iss 2, p 638 (2018)
International Journal of Molecular Sciences; Volume 19; Issue 2; Pages: 638
ISSN: 1422-0067
Popis: Agrobacterium tumefaciens can genetically transform various eukaryotic cells because of the presence of a resident tumor-inducing (Ti) plasmid. During infection, a defined region of the Ti plasmid, transfer DNA (T-DNA), is transferred from bacteria into plant cells and causes plant cells to abnormally synthesize auxin and cytokinin, which results in crown gall disease. T-DNA and several virulence (Vir) proteins are secreted through a type IV secretion system (T4SS) composed of T-pilus and a transmembrane protein complex. Three members of Arabidopsis reticulon-like B (RTNLB) proteins, RTNLB1, 2, and 4, interact with VirB2, the major component of T-pilus. Here, we have identified that other RTNLB proteins, RTNLB3 and 8, interact with VirB2 in vitro. Root-based A. tumefaciens transformation assays with Arabidopsis rtnlb3, or rtnlb5-10 single mutants showed that the rtnlb8 mutant was resistant to A. tumefaciens infection. In addition, rtnlb3 and rtnlb8 mutants showed reduced transient transformation efficiency in seedlings. RTNLB3- or 8 overexpression transgenic plants showed increased susceptibility to A. tumefaciens and Pseudomonas syringae infection. RTNLB1-4 and 8 transcript levels differed in roots, rosette leaves, cauline leaves, inflorescence, flowers, and siliques of wild-type plants. Taken together, RTNLB3 and 8 may participate in A. tumefaciens infection but may have different roles in plants.
Databáze: OpenAIRE
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