| Autor: |
Stefano D’Alessandro, Florent Velay, Régine Lebrun, Marwa Mehrez, Shanna Romand, Rim Saadouni, Céline Forzani, Sylvie Citerne, Marie-Hélène Montané, Christophe Robaglia, Benoît Menand, Christian Meyer, Ben Field |
| Přispěvatelé: |
Università degli Studi di Padova = University of Padua (Unipd), Institut de Biosciences et Biotechnologies d'Aix-Marseille (ex-IBEB) (BIAM), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Biologie végétale et microbiologie environnementale - UMR7265 (BVME), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Luminy Génétique et Biophysique des Plantes (LGBP), Institut de Microbiologie de la Méditerranée (IMM), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), University of Tunis El Manar, Institut Jean-Pierre Bourgin (IJPB), AgroParisTech-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), ANR-17-CE13-0005,G4PLAST,Mecanismes d'acclimation aux stress par la voie conservée de signalisation du (p)ppGpp dans le chloroplaste.(2017), ANR-22-CE20-0033,TARGET_G4P,Identification des cibles et de la régulation de la voie ppGpp chez les plantes.(2022) |
| Rok vydání: |
2023 |
| Předmět: |
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| DOI: |
10.1101/2023.05.05.539554 |
| Popis: |
Chloroplasts are the powerhouse of the plant cell, yet they are resource-intensive and will cause photooxidative damage if their activity overshoots the demands of growth. The adjustment of chloroplast activity to match growth is therefore vital for stress acclimation. Here we identify a novel post-translational mechanism linking the conserved eukaryotic TOR kinase that promotes growth and the guanosine tetraphosphate (ppGpp) signaling pathway of prokaryotic origin that regulates chloroplast activity, and photosynthesis in particular. We show that RelA SpoT Homologue 3 (RSH3), a nuclear-encoded chloroplastic enzyme responsible for ppGpp biosynthesis, interacts directly with the TOR complex via a plant-specific N-terminal region (NTR) which is hyper-phosphorylated in a TOR-dependent manner. Downregulation of TOR activity reduces NTR phosphorylation, enhances ppGpp synthesis by RSH3, and causes a ppGpp-dependent decrease in photosynthetic capacity. Altogether we demonstrate that the TOR-RSH3 signaling axis is a novel and direct post-translational mechanism that allows chloroplast activity to be matched with plant growth, setting a new precedent for the regulation of organellar function by TOR.One sentence summaryThe TOR kinase post-translationally controls guanosine tetraphosphate signaling to regulate plant photosynthetic activity. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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