Canine Placenta Recellularized Using Yolk Sac Cells with Vascular Endothelial Growth Factor
| Autor: | Maria Angélica Miglino, Rose Eli Grassi Rici, Ana Claudia Oliveira Carreira, Paula Fratini, Gustavo de Sá Schiavo Matias, Nathia Nathaly Rigoglio |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Scaffold placenta extracellular matrix yolk sac cells lcsh:Medicine Regenerative medicine Article General Biochemistry Genetics and Molecular Biology Extracellular matrix 03 medical and health sciences chemistry.chemical_compound Laminin medicine Yolk sac lcsh:QH301-705.5 BIOMATERIAIS Decellularization biology Chemistry lcsh:R recellularization VEGF Cell biology Vascular endothelial growth factor Fibronectin 030104 developmental biology medicine.anatomical_structure lcsh:Biology (General) biology.protein decellularization |
| Zdroj: | BioResearch Open Access, Vol 7, Iss 1, Pp 101-106 (2018) Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP BioResearch Open Access |
| ISSN: | 2164-7860 2164-7844 |
| Popis: | Regenerative medicine has been growing because of the emergent need for tissues/organs for transplants and restorative surgeries. Biological scaffolds are important tools to try to solve this problem. The one used in this reserach was developed by an acellular biological scaffold from canine placenta with a rich source of cellular matrix. After decellularization, the cellular matrix demonstrated structural preservation with the presence of important functional proteins such as collagen, fibronectin, and laminin. We used cells transduced with vascular endothelial growth factor (VEGF) to recellularize this scaffold. It was succeeded by seeding the cells in nonadherent plaques in the presence of the sterelized placenta scaffold. Cells were adhered to the scaffold when analyzed by immunocytochemistry and scanning electron microscopy, both showing sprouting of yolk sac VEGF (YSVEGF) cells. This recellularized scaffold is a promissory biomaterial for repairing injured areas where neovascularization is required. |
| Databáze: | OpenAIRE |
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