Ultrastructural Characterization of SARS Coronavirus
| Autor: | Bettina Bankamp, Kathleen M. Tatti, Thomas G. Ksiazek, William J. Bellini, William W. Lee, Cynthia S. Goldsmith, James A. Comer, Sherif R. Zaki, Pierre E. Rollin, Paul A. Rota |
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| Jazyk: | angličtina |
| Rok vydání: | 2004 |
| Předmět: |
Microbiology (medical)
Epidemiology viruses immunogold techniques coronavirus lcsh:Medicine SARS virus severe acute respiratory syndrome Biology medicine.disease_cause Virus Inclusion bodies Inclusion Bodies Viral emerging infectious diseases lcsh:Infectious and parasitic diseases Viral Proteins Chlorocebus aethiops medicine Animals Humans replication complex lcsh:RC109-216 Microscopy Immunoelectron Vero Cells Coronavirus electron microscopy Virus Assembly Research lcsh:R Immunogold labelling medicine.disease ultrastructure Virology Microscopy Electron Pneumonia Infectious Diseases Severe acute respiratory syndrome-related coronavirus Cytoplasm Ultrastructure Vero cell RNA Viral in situ hybridization Bronchoalveolar Lavage Fluid |
| Zdroj: | Emerging Infectious Diseases, Vol 10, Iss 2, Pp 320-326 (2004) Emerging Infectious Diseases |
| ISSN: | 1080-6059 1080-6040 |
| Popis: | Severe acute respiratory syndrome (SARS) was first described during a 2002–2003 global outbreak of severe pneumonia associated with human deaths and person-to-person disease transmission. The etiologic agent was initially identified as a coronavirus by thin-section electron microscopic examination of a virus isolate. Virions were spherical, 78 nm in mean diameter, and composed of a helical nucleocapsid within an envelope with surface projections. Herein, we show that infection with the SARS-associated coronavirus resulted in distinct ultrastructural features: double-membrane vesicles, nucleocapsid inclusions, and large granular areas of cytoplasm. These three structures and the coronavirus particles were shown to be positive for viral proteins and RNA by using ultrastructural immunogold and in situ hybridization assays. In addition, ultrastructural examination of a bronchiolar lavage specimen from a SARS patient showed numerous coronavirus-infected cells with features similar to those in infected culture cells. Electron microscopic studies were critical in identifying the etiologic agent of the SARS outbreak and in guiding subsequent laboratory and epidemiologic investigations. |
| Databáze: | OpenAIRE |
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