Scoparone Induces Expression of Pluripotency Transcription Factors SOX2 and NANOG in Dermal Papilla Cells
| Autor: | Pichit Suvanprakorn, Boonchoo Sritularak, Nattha Suwanprakorn, Pithi Chanvorachote, Thitiporn Tongyen |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Homeobox protein NANOG
Cancer Research Apoptosis General Biochemistry Genetics and Molecular Biology chemistry.chemical_compound Western blot SOX2 Coumarins medicine Humans MTT assay Transcription factor Pharmacology medicine.diagnostic_test SOXB1 Transcription Factors Stem Cells Nanog Homeobox Protein Hair follicle Up-Regulation Cell biology Scoparone medicine.anatomical_structure chemistry embryonic structures Stem cell Hair Follicle Research Article |
| Zdroj: | In Vivo |
| ISSN: | 1791-7549 0258-851X |
| Popis: | Background/aim: Dermal papilla cells (DPCs) regulate hair follicle development. We aimed to investigate the effect of scoparone from Dendrobium densiflorum on DPCs in the induction of stem cell properties and pluripotency-related proteins. Materials and methods: DPC viability was evaluated by the MTT assay. Apoptosis or necrosis of DPCs was determined by Hoecsht33342/PI nuclear staining analysis. Expression of OCT4, NANOG and SOX2 genes was determined using Real-Time Polymerase Chain Reaction (PCR). Immunocytochemistry and western blot analysis were performed to determine pluripotency related proteins. Results: Scoparone increased the expression of pluripotency related transcription factors SOX2 and NANOG, while it had minimal effects on OCT4 levels. Scoparone exerted its stemness-enhancing activity through the up-regulation of Akt-dependent inhibition of GSK3β, resulting in increased cellular levels of β-catenin. Conclusion: Our results show a potential novel activity and mechanism of action of scoparone on human DPCs that could facilitate the development of hair enrichment approaches. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|