Alterations induced by citrinin in cultured kidney cells
| Autor: | Dorly de Freitas Buchi, Annibal P. Campello, Maria Benigna M. Oliveira, M. L. W. Kluppel, Generoso M. Chagas |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Programmed cell death
animal structures Time Factors Physiology Hamster Mitochondrion Biology Kidney chemistry.chemical_compound Cricetinae Baby hamster kidney cell medicine Cell Adhesion Animals Cytotoxicity Molecular Biology Cells Cultured Dose-Response Relationship Drug Cell Membrane Cell Biology General Medicine Molecular biology Citrinin Mitochondria Microscopy Electron medicine.anatomical_structure chemistry Biochemistry Cell culture Oxidation-Reduction |
| Zdroj: | Cell structure and function. 19(2) |
| ISSN: | 0386-7196 |
| Popis: | The cytotoxicity of citrinin was evaluated in an established cell line of baby hamster kidney cells. The primary effect of the mycotoxin was on the adherence of the cells to the culture bottles. Microscopic evaluation of morphological alterations indicated that the cells which were originally elongated and flattened, became swollen and round. Electron microscopic examination showed that citrinin (0.1, 0.5 and 1.0 mM) incubated for 10 hours with cultured cells, promoted drastic alterations of normal mitochondria, with swelling and cell death. Transplasma membrane redox system is inhibited by citrinin (81%). This effect is dependent not only on the toxin concentration, but also on the time of exposure to the cells. |
| Databáze: | OpenAIRE |
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